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采用不同浓度抗小鼠ＣＤ３ 复合物单抗刺激幼龄小鼠胸腺细胞，培养后分别在不同时间用流式细胞仪检测胸腺细胞的凋亡情况。结果表明，在ＣＤ３ 单抗诱导幼龄小鼠胸腺细胞４ 小时后，流式细胞仪即可测出凋亡细胞特有的ＡＰ峰。本项研究提示，用ＣＤ３ 单抗刺激未成熟胸腺细胞可以通过内源性的凋亡途径引起细胞死亡。未成熟Ｔ 细胞通过ＴＣＲ－ＣＤ３ 复合物与自身抗原接合激活上述过程可能与克隆清除的形成机制及自我耐受有关。
The thymocytes of young mice were stimulated with different concentrations of anti-mouse CD3 complex monoclonal antibody, and the apoptosis of thymocytes was detected by flow cytometry at different times after culture. The results showed that the AP peak specific to apoptotic cells was detected by flow cytometry 4 hours after the CD3 monoclonal antibody induced thymocytes in young mice. This study suggests that stimulation of immature thymocytes with CD3 mAb can cause cell death via the endogenous apoptotic pathway. Activation of immature T cells by TCR-CD3 complex with autoantigen may be related to the formation of clonal clearance and self-tolerance.