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目的 本研究旨在评价血清脂肪酸结合蛋白 (FABP)定量检测对急性心肌梗死 (AMI)患者早期诊断价值。方法 采用酶联免疫吸附试验一步夹心法对 2 0名健康体检者、2 6例AMI患者进行血清FABP检测 ,同时测定心肌肌钙蛋白T(cTnT)、肌酸激酶同工酶MB(CK MB) ,并对其中 9例AMI胸痛发作后 6h内入院患者血清进行动态分析。结果 2 0名健康体检者FABP、cTnT、CK MB血清浓度分别为 (1 8± 0 9) μg/L、(0 16± 0 10 ) μg/L、(15 2± 3 8)U/L ,2 6例AMI患者血清浓度分别为 (38 6±2 1 8) μg/L、(2 5± 2 4) μg/L和 (90 5± 83 5 )U/L ,阳性率为 10 0 %、84 5 %和 80 8%。 9例动态观察的患者中 ,有 4例 1h内入院时血清中FABP浓度开始升高 [(14 1± 4 1) μg/L],9例患者血清升高达峰值时间为 (7 0± 3 2 )h ,比cTnT、CK MB早 (P <0 0 0 1) ,2 4～ 48h恢复正常。结论 FABP可作为急性心肌梗死早期诊断或排除的血清标志物
Objectives This study was designed to evaluate the value of quantitative detection of serum fatty acid binding protein (FABP) in the early diagnosis of patients with acute myocardial infarction (AMI). Methods Serum FABP was detected by enzyme-linked immunosorbent assay (ELISA) in 20 healthy subjects and 26 AMI patients. Cardiac troponin T (cTnT), creatine kinase MB (CK MB) , And 9 cases of patients with AMI chest pain within 6 hours after the onset of the dynamic analysis of hospitalized patients. Results The serum concentrations of FABP, cTnT and CK MB were (18 ± 0 9) μg / L, (0 16 ± 0 10) μg / L and (15 2 ± 3 8) U / The serum concentrations of 26 patients with AMI were (386 ± 2118) μg / L, (25 ± 24) μg / L and (905 ± 83 5) U / L, 84 5% and 80 8%. Of the 9 dynamically observed patients, 4 had a rise in serum FABP concentrations within 1 h ([14 1 ± 4 1] μg / L], and 9 had elevated serum levels (70 ± 32 ) h, which was earlier than cTnT and CK MB (P <0.01), returned to normal after 24-48h. Conclusion FABP can be used as a serum marker for early diagnosis or exclusion of acute myocardial infarction