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目的探讨Bell麻痹患儿外周血CD19+B、CD20+B淋巴细胞(CD19+B、CD20+B)、CD3+T淋巴细胞(CD3+T)的表达及治疗后改变情况,分析其与症状改善程度的相关性。方法选择2011年1月至2014年5月在中山大学孙逸仙纪念医院儿童神经专科就诊的Bell麻痹患儿30例,应用流式细胞仪检测患儿外周血CD19+B、CD20+B、CD3+T淋巴细胞的表达情况。正常对照组为同院体检健康儿童30名,年龄和性别构成比例与病例组差异无统计学意义(P>0.05)。结果 30例患儿CD19+B细胞和CD20+B细胞比例分别为(38.02%±10.00%)、(36.02%±9.80%),明显高于正常对照组(16.43%±3.99%)、(15.98%±3.95%),差异有统计学意义(P<0.05)。30例患儿治疗20 d后,23例症状改善,7例症状无明显改善。症状改善组治疗后CD19+B细胞和CD20+B细胞比例分别为(16.54%±3.06%)、(18.33%±3.33%),较治疗前(39.76%±9.58%)、(38.25%±9.73%)明显降低,差异有统计学意义(P<0.05);症状无明显改善组分别为(39.06%±3.66%)、(38.07%±2.36%),较治疗前(39.45%±3.78%)、(39.47%±2.63%)差异无统计学意义(P>0.05)。结论 Bell麻痹患儿存在B淋巴细胞免疫功能异常;外周血CD19+B细胞、CD20+B细胞比例改变可能与Bell麻痹患儿症状改善程度存在一定相关性。
Objective To investigate the expression of CD19 + B, CD20 + B lymphocytes (CD19 + B, CD20 + B) and CD3 + T lymphocytes (CD3 + T) in children with Bell palsy and their changes after treatment, Degree of relevance. Methods From January 2011 to May 2014, 30 children with Bell’s palsy admitted to the Children’s Neurological Department of Sun Yat-sen Memorial Hospital of Sun Yat-sen University were enrolled in this study. The levels of CD19 + B, CD20 + B and CD3 + T in peripheral blood were measured by flow cytometry Lymphocyte expression. The normal control group was 30 healthy children in the same hospital. There was no significant difference between the age and sex composition and the case group (P> 0.05). Results The percentages of CD19 + B cells and CD20 + B cells in the 30 children were (38.02% ± 10.00%) and (36.02% ± 9.80%) respectively, significantly higher than those in the normal control group (16.43% ± 3.99%, 15.98% ± 3.95%), the difference was statistically significant (P <0.05). After 30 days of treatment, the symptoms improved in 23 cases and no significant improvement in 7 cases. The percentages of CD19 + B cells and CD20 + B cells in the symptom improvement group were (16.54% ± 3.06%) and (18.33% ± 3.33%, respectively) compared with those before treatment (39.76% ± 9.58%, 38.25% ± 9.73% ) Was significantly lower than that before treatment (39.45% ± 3.78%, P <0.05), and the difference was not statistically significant (39.06% ± 3.66%, 38.07% ± 2.36% 39.47% ± 2.63%) with no significant difference (P> 0.05). Conclusions The immunological function of B lymphocytes in children with Bell palsy is abnormal. The changes of the proportion of CD19 + B cells and CD20 + B cells in peripheral blood may be related to the improvement of symptoms in children with Bell palsy.