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目的探讨四氯化碳(CCl4)一过性处理对巴马香猪肠道菌群的影响,评估CCl4诱导处理的肝损伤动物模型能否用于肠道微生态研究。方法选取3头雄性巴马香猪,一次性按每千克体重腹腔注射0.25 ml[0.25ml/(kg.bw)]40%CCl4橄榄油溶液。于注射12 h之后的第1、2、3、4、7和10天连续采集粪便,以注射CCl4溶液之前所采集粪便样作对照。分别提取粪样总DNA,采用变性梯度凝胶电泳技术(DGGE)对CCl4一过性处理后巴马香猪肠道菌群的多样性变化进行动态监测。结果PCR-DGGE法分析表明,巴马香猪肠道菌群组成在注射CCl4前后差异无显著性(P>0.05)。相似性聚类分析结果表明,菌群组成在注射CCl4前后相似度在65%以上。结论CCl4一过性处理后巴马香猪肠道菌群组成未发生显著变化。表明CCl4处理诱导的肝损伤动物模型可以用来研究相关疾病与肠道菌群之间的关系。
Objective To investigate the effects of carbon tetrachloride (CCl4) transient treatment on the intestinal flora of Bama xiang pigs and to evaluate whether CCl4 - induced liver injury animal models can be used for gut microflora research. Methods Three male Bama pigs were selected and intraperitoneally injected with 0.25 ml [0.25ml / (kg.bw)] 40% CCl4 olive oil per kilogram of body weight. Stool samples were collected continuously on days 1, 2, 3, 4, 7, and 10 after injection for 12 h, and stool sample collected before injection of CCl 4 solution was used as a control. The total DNA of fecal sample was extracted respectively and the diversity of intestinal flora of Bamar pig after transient treatment with CCl4 was dynamically monitored by denaturing gradient gel electrophoresis (DGGE). Results The results of PCR-DGGE showed that there was no significant difference in the intestinal flora composition of Bama Xiang pigs before and after injection of CCl4 (P> 0.05). The results of similarity clustering showed that the similarity of the flora before and after injection of CCl4 was over 65%. Conclusion There was no significant change in the intestinal flora of Bama Xiang pig after transient treatment with CCl4. The animal model of liver injury induced by CCl4 treatment can be used to study the relationship between the related diseases and intestinal flora.