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[目的]探讨前交叉韧带损伤后尿激酶型纤溶酶原激活物形成变化。[方法]20只新西兰兔一期建立单侧前交叉韧带损伤模型。损伤术前、术后2、4和8周时采用ELISA检测血浆中尿激酶型纤溶酶原激活物含量变化。损伤术后8周时采用HE染色和免疫组织化学检测关节内组织病理变化和尿激酶型纤溶酶原激活物表达情况。[结果]前交叉韧带损伤后血浆尿激酶型纤溶酶原激活物含量变化明显,术后2周时尿激酶型纤溶酶原激活物含量显著增高(P<0.05)。术后8周时组织学检测显示滑膜、软骨和前交叉韧带呈病理改变,组织内尿激酶型纤溶酶原激活物广泛表达、阳性细胞数量增多(P<0.01)。[结论]前交叉韧带损伤后关节腔内多种组织尿激酶型纤溶酶原激活物表达异常,血浆尿激酶型纤溶酶原激活物含量变化明显。
[Objective] To investigate the changes of urokinase-type plasminogen activator after anterior cruciate ligament injury. [Method] The unilateral cruciate ligament injury model was established in 20 New Zealand rabbits. The levels of urokinase-type plasminogen activator in plasma were detected by ELISA before, 2, 4 and 8 weeks after injury. At 8 weeks after injury, the histopathological changes and the expression of urokinase-type plasminogen activator were detected by HE staining and immunohistochemistry. [Results] The content of urokinase-type plasminogen activator significantly changed after anterior cruciate ligament injury, and the content of urokinase-type plasminogen activator was significantly increased at 2 weeks after operation (P <0.05). Histological examination showed synovium, cartilage and anterior cruciate ligament showed pathological changes at 8 weeks after operation. Urokinase-type plasminogen activator was widely expressed and the number of positive cells increased (P <0.01). [Conclusion] The expression of urokinase-type plasminogen activator in various tissues of articular cavity after anterior cruciate ligament injury is abnormal, and the content of plasma urokinase-type plasminogen activator significantly changes.