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探讨克服肿瘤细胞多药耐药（ＭＤＲ１）性的方法，提高化疗效果。本文采用多药耐药反义基因（ＭＤＲ１－ＲＳＰＳ－ＯＤＮ）逆转Ｋ５６２／ＡＤＭ肿瘤细胞的ＭＤＲ１，诱导肿瘤细胞凋亡，发现ＭＤＲ１－ＡＳＰＳ－ＯＤＮ诱导Ｋ５６２／ＡＤＭ细胞株细胞产生大量ＤＮＡ断片，ＦＡＣＳ检测发现几乎全部ＭＲＤ＋Ｋ５６２／ＡＤＭ细胞发生凋亡。其结果表明ＤＭＤＲ１－ＡＳＰＳ－ＯＤＮ能有效、特异地抑制ＭＤＲ１基因表达，逆转肿瘤细胞的ＭＤＲ１，促进阿霉素诱导ＭＤＲ＋１Ｋ５６２／ＡＤＭ细胞凋亡，为其临床应用提供理论依据
To explore ways to overcome the multidrug resistance (MDR1) of tumor cells and increase the effect of chemotherapy. In this study, multidrug resistance antisense gene (MDR1-RSPS-ODN) was used to reverse MDR1 in K562/ADM tumor cells and induce tumor cell apoptosis. It was found that MDR1-ASPS-ODN induces a large number of DNA fragments in K562/ADM cell lines, FACS Almost all MRD+K562/ADM cells were found to undergo apoptosis. The results show that DMDR1-ASPS-ODN can effectively and specifically inhibit the expression of MDR1 gene, reverse the MDR1 of tumor cells, and promote the apoptosis of MDR+1K562/ADM cells induced by doxorubicin, providing a theoretical basis for its clinical application.