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本文采用光敏生物素法标记人乳头瘤病毒(HPV)16,18型DNA探针,进行斑点杂交检测宫颈癌组织中HPV16、18DNA相关序列。检测结果为,18例宫项癌组织中5例HPV16杂交阳性(27.78%);14例HPV18杂交阳性(77.8%)。这表明宫颈癌的发生和HPV感染密切相关。
In this paper, HPV 16 and 18 DNA probes were labeled with biotinylated biotin for detection of HPV16 and 18 DNA-related sequences in cervical cancer by dot blot hybridization. The results showed that in 18 cases of cervical cancer tissues, 5 cases of HPV16 positive hybridization (27.78%); 14 cases of HPV18 hybridization positive (77.8%). This shows that the occurrence of cervical cancer and HPV infection are closely related.