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目的：探讨肿瘤坏死因子（TN－α）对人气道平滑肌细胞内皮素转换酶－1（ECE－1）基因表达的影响及体外反义内皮素转换酶－1寡聚核苷酸（ECE—1oDNS）是否可拮抗TNF－α诱导人气道平滑肌细胞ECE－1表达分泌．方法：高体培养人气过平滑肌细胞共导入由阳离子脂质体Lipofectin携载的不同浓度反义ECEoDNS，用RTPCR观察其对前炎症因子TNF-α诱导人气道平滑肌细胞ECE基因表达的影响．结果：TNF－α可刺激ECE-1mRNA表达．ECE－1mRNA由0．1318±0．0318升至0．2277±0．0150（t=4．724，P＜0．01）；导入反义ECEoDNS后，ECE－1mRNA表达受到抑制，抑制率分别为34．74％±11．6％及29．24％±4.96％．结论：反义ECE－1oDNS能拮抗TNF－α诱导人气道平滑肌细胞的ECE—1mRNA表达，为从基因治疗角度探讨控制气道局反应提供新线索．
Objective: To investigate the effects of tumor necrosis factor-α (TNF-α) on the expression of endothelin-converting enzyme-1 (ECE-1) gene in human airway smooth muscle cells and the effect of antisense endothelin-converting enzyme-1 oligonucleotide ) Could antagonize TNF-α-induced secretion of ECE-1 in human airway smooth muscle cells. Methods: Human antisense ECEoDNS transfected with Lipofectin was introduced into human peritumoral smooth muscle cells (VSMCs). The effects of TNF-α on ECE gene expression in human airway smooth muscle cells were observed by RTPCR. Results: TNF-α stimulated ECE-1 mRNA expression. ECE-1 mRNA increased from 0.1318 ± 0.0318 to 0.2277 ± 0.0150 (t = 4.724, P <0.01). After the antisense ECEoDNS was introduced, the expression of ECE-1 mRNA was inhibited and the inhibition rates were 34.74% ± 11.6% and 29.24% ± 4.96%. CONCLUSION: Antisense ECE-1oDNS can antagonize TNF-α-induced ECE-1mRNA expression in human airway smooth muscle cells and provide new clues for exploring ways to control airway response from gene therapy.