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目的探究红豆越橘提取物对慢性应激损伤小鼠认知功能的影响。方法将雄性昆明小鼠随机分为空白对照组,应激模型组,氟西汀(4.4 mg·kg-1·d-1)阳性对照组及红豆越橘提取物低、中、高(50,100和200 mg·kg-1·d-1)剂量组,给药组采取连续慢性应激,并灌胃给药18 d,通过水迷宫实验测定小鼠认知记忆能力,测定与抗氧化功能相关的小鼠血清和脑组织中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量,测定与应激损伤相关的小鼠血清和脑组织中神经递质去甲肾上腺素(NE)、5-羟基色胺(5-HT),糖皮质激素(GC)和乙酰胆碱酯酶(ACh E)活性。结果红豆越橘提取物能够改善应激模型小鼠的认知记忆能力;增加脑组织中SOD、GSH-Px酶活性,降低应激损伤产物MDA的含量;同时抑制血清中GC的升高及Ach E的激活,促进血清及脑组织中5-HT、NE含量的升高。结论红豆越橘提取物能够改善应激损伤小鼠的学习认知能力,其可能机制为通过增强抗氧化能力来降低氧化损伤并改善应激造成的神经递质水平紊乱。
Objective To investigate the effect of bilberry extract on cognitive function in chronic stress-induced injury in mice. Methods Male Kunming mice were randomly divided into control group, stress model group, positive control group with fluoxetine (4.4 mg · kg -1 · d -1) and low, medium and high 200 mg · kg-1 · d-1). The rats in the treatment group were given continuous chronic stress and were administered intragastrically for 18 days. The water maze test was used to measure the cognitive memory in mice, and the anti-oxidative function was determined The activity of serum superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and the content of malondialdehyde (MDA) in serum and brain of mice were measured, and the serum and brain Neurotransmitters norepinephrine (NE), 5-hydroxytryptamine (5-HT), glucocorticoid (GC) and acetylcholinesterase (AChE) activity in the tissues. Results Bilberry extract could improve the cognition and memory ability of stress-induced model mice, increase the activities of SOD and GSH-Px in brain tissues, decrease the content of MDA in stress injury products, inhibit the increase of GC in serum and Ach E activation, and promote serum and brain tissue 5-HT, NE content increased. Conclusion Bilberry extract can improve the learning and cognitive abilities in stress-injured mice. Its possible mechanism is to reduce the oxidative damage and improve the neurotransmitter levels caused by stress by increasing the antioxidant capacity.