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目的研究等位基因DC-SIGN/DC-SIGNR在人类免疫缺陷病毒Ⅰ型(HIV-1)感染者与HIV-1阴性吸毒人群中的分布,筛选出HIV-1抗性基因。方法选择深圳市戒毒所HIV抗体阴性戒毒者的样本257份,HIV-1感染者的样本220份。提取外周血基因组DNA,对DC-SIGN/DC-SIGNR颈区基因进行特异性聚合酶链反应(PCR)扩增,PCR扩增产物行琼脂糖凝胶电泳检测和基因序列测定分析。结果通过统计学分析发现,DC-SIGNR颈区中杂合子7/5、7/6和7/7在HIV阴性吸毒者中出现的频率为13.33%、7.50%、49.4%,在HIV-1感染者中出现的频率为11.05%、4.65%、51.74%,两者无明显差异。说明DC-SIGN在吸毒者与HIV-1感染者中的基因型及等位基因的分布无明显差异。结论深圳市吸毒人群中DC-SIGNR和DC-SIGN基因型分布和等位基因出现频率,与HIV-1感染者无显著性差异,推测DC-SIGNR和DC-SIGN基因多态性对HIV-1易感性无明显影响。
Objective To study the distribution of allele DC-SIGN / DC-SIGNR in human immunodeficiency virus type 1 (HIV-1) and HIV-1 negative drug users and screen HIV-1 resistance genes. Methods A total of 257 HIV-negative drug addicts from Shenzhen City Drug Treatment Center and 220 HIV-1-infected persons were selected. Genomic DNA was extracted from the peripheral blood to amplify the specific region of the DC-SIGN / DC-SIGNR neck region by polymerase chain reaction (PCR). The PCR products were analyzed by agarose gel electrophoresis and sequence analysis. Results The frequency of heterozygotes 7/5, 7/6 and 7/7 in DC-SIGNR neck region was 13.33%, 7.50% and 49.4% respectively in HIV-1 infection The frequency of occurrence was 11.05%, 4.65%, 51.74%, no significant difference between the two. Indicating that there was no significant difference in the distribution of genotypes and alleles of DC-SIGN among drug addicts and HIV-1 infected persons. Conclusion The distribution of DC-SIGNR and DC-SIGN genotypes and the frequency of alleles in drug addicts in Shenzhen are not significantly different from those in HIV-1 infected persons. It is presumed that the polymorphisms of DC-SIGNR and DC-SIGN genes are negatively correlated with HIV-1 Susceptibility no significant effect.