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OBJECTIVE:To investigate effects of Saikosaponin D(SSd) on syndecan-2,matrix metalloproteinases(MMPs) and tissue inhibitor of metalloproteinases-2(TIMP-2) in livers of rat with hepatocellular carcinoma(HCC).METHODS:Male SD rats were divided into control(n=10),model(n=20) and SSd(n=20) groups,and model and SSd groups given intragastric 0.2%(w/v) N-diethylnitrosamine to induce HCC.SSd group received 0.03%(w/v) SSd in saline.Liver samples were analysed immunohistochemically for syndecan-2,MMP-2,MMP-13 and TIMP-2 at 16 weeks.RESULTS:The model group had more malignant nodules than the SSd group;all model-group HCC cells were grade III;SSd-group HCC cells were grades I-II.Controls showed normal hepatic cell phenotypes and no syndecan-2 + staining.Syndecan-2 + staining was greater in the model group(35.2%,P≤0.001) than in controls or the SSd group(16.5%,P ≤ 0.001).The model group had more intense MMP-2 + staining than controls(0.37 vs 0.27,P≤0.01) or the SSd group(0.31 vs 0.37,P≤0.05);and higher MMP-13 + staining(72.55%) than in controls(12.55%,P≤0.001) and SSd group(20.18%,P≤0.01).The model group also had more TIMP-2 + staining(57.2%) than controls(20.9%,P≤0.001) and SSd group(22.7%,P≤0.001).Controls and SSd group showed no difference in TIMP-2 + rates.CONCLUSION:SSd inhibited HCC development,and downregulated expression of syndecan-2,MMP-2,MMP-13 and TIMP-2 in rat HCC liver tissue.
OBJECTIVE: To investigate effects of Saikosaponin D (SSd) on syndecan-2, matrix metalloproteinases (MMPs) and tissue inhibitor of metalloproteinases-2 (TIMP-2) in livers of livers with hepatocellular carcinoma (HCC) The models were divided into control (n = 10), model (n = 20) and SSd (n = 20) groups, and model and SSd groups given intragastric 0.2% (w / v) N-diethylnitrosamine to induce HCC.SSd group received 0.03% (w / v) SSd in saline. Liver samples were analyzed for immunohistochemically for syndecan-2, MMP-2, MMP-13 and TIMP-2 at 16 weeks .RESULTS: The model group had more malignant nodules than the SSd group; all model -group HCC cells were grade III; SSd-group HCC cells were grades I-II. Controls showed hepatic cell phenotypes and no syndecan-2 + staining. Syndecan-2 + staining was greater in the model group (35.2% 0.001) than in controls or the SSd group (16.5%, P ≤ 0.001) .The model group had more intense MMP-2 + staining than controls (0.37 vs 0.27, P ≦ 0.01) or the SSd group (0.31 vs 0.37, P ≤0.05) (12.5%, P≤0.001) and SSd group (20.18%, P≤0.01). The model group also had more TIMP-2 + staining (57.2%) than in controls than controls (20.9%, P≤0.001) and SSd group (22.7%, P≤0.001). Control and SSd group showed no difference in TIMP-2 + rates.CONCLUSION: SSd inhibited HCC development, and downregulated expression of syndecan- 2 , MMP-2, MMP-13 and TIMP-2 in rat HCC liver tissue.