论文部分内容阅读
目的研究RNPC1基因对胃癌细胞MGC-823增殖、迁移及侵袭的影响。方法胃癌细胞MGC-823中构建RNPC1基因过表达(RNPC1组)及短发夹RNA干扰RNPC1基因表达(shRNPC1组),另设空白对照组(NC组)以及阴性对照组(SCR组)。采用实时荧光定量PCR和Western blot检测RNPC1mRNA及蛋白表达水平,CCK-8法检测细胞增殖能力,划痕实验和Transwell小室实验检测细胞迁移和侵袭能力。结果shRNPC1组RNPC1 mRNA及蛋白相对表达量均低于SCR组,而RNPC1组RNPC1mRNA及蛋白相对表达量均高于NC组(P<0.01)。shRNPC1组细胞增殖能力弱于SCR组,而RNPC1组细胞增殖能力强于NC组(P<0.01)。shRNPC1组细胞迁移和侵袭能力弱于SCR组,而RNPC1组细胞迁移和侵袭能力均强于NC组(P<0.01)。结论 RNPC1基因过表达能增强MGC-823细胞增殖、迁移及侵袭能力,可为临床治疗提供新的作用靶点。
Objective To study the effect of RNPC1 gene on proliferation, migration and invasion of gastric cancer cell line MGC-823. Methods RNPC1 gene overexpression (RNPC1) and short hairpin RNA (RNAi) were transfected into gastric cancer cell line MGC-823 to interfere with the gene expression of RNPC1 (shRNPC1 group), and blank control group (NC group) and negative control group (SCR group) Real-time PCR and Western blot were used to detect the expression of RNPC1 mRNA and protein. CCK-8 method was used to detect the proliferation of cells. Scratch assay and Transwell chamber assay were used to detect the migration and invasion of cells. Results The relative expression of RNPC1 mRNA and protein in shRNPC1 group was lower than that in SCR group. The relative expression of RNPC1 mRNA and protein in RNPC1 group was higher than that in NC group (P <0.01). The proliferation of shRNPC1 group was weaker than that of SCR group, while the proliferation of RNPC1 group was stronger than that of NC group (P <0.01). The migration and invasion ability of shRNPC1 group was weaker than that of SCR group, while the migration and invasion ability of RNPC1 group were stronger than that of NC group (P <0.01). Conclusion RNPC1 gene overexpression can enhance the proliferation, migration and invasion of MGC-823 cells, which may provide a new therapeutic target for clinical treatment.