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目的分析四川省2004年7起因聚餐暴发霍乱疫情的分离菌株之间,及其与常规监测中从外环境、水产品中分离的霍乱弧菌之间的分子分型特征和遗传相关性,查找霍乱传染来源。方法利用聚合酶链反应检测霍乱毒力基因(ctxAB),用脉冲场凝胶电泳(PFGE)对菌株进行分子分型,所得结果以BioNumerics V4.0软件UPGMA方法进行聚类分析。结果所试72株O139群霍乱弧菌中所有4株从河水分离的菌株ctxAB阴性,其余均具有ctxAB,为产毒株。对其中的67株菌以NotⅠ酶切后PFGE可分为16个型别。从甲鱼分离的O139群霍乱弧菌与同期流行的O139群霍乱弧菌优势PFGE型别一致,并且也为产毒株。7起暴发中分离的菌株型别各不相同。结论2004年四川省O139霍乱暴发感染来源复杂,提示并非因为菌株在该地持续存在而引起,但甲鱼可能是2004年度霍乱聚餐暴发的主要传染来源之一。
OBJECTIVE: To analyze the molecular typing and genetic correlation between seven isolates isolated from cholera outbreaks in Sichuan Province in 2004 and V. cholerae isolated from external environment and aquatic products in routine monitoring, Source of infection. Methods The cholera virulence gene (ctxAB) was detected by polymerase chain reaction (PCR) and the strains were genotyped by pulsed-field gel electrophoresis (PFGE). The results were clustered by UPGMA with BioNumerics V4.0 software. Results All the 4 strains isolated from Vibrio cholerae of 72 strains of O139 were negative for ctxAB and the others were ctxAB. Of 67 strains of NotI digested PFGE can be divided into 16 types. Vibrio cholera O139 isolated from turtles was consistent with the predominant PFGE type of V. cholerae O139 circulating in the same period and was also an anaerobic producer. The strains isolated in the seven outbreaks differed. Conclusion The sources of outbreaks of cholera outbreak in O139 in Sichuan Province in 2004 are complex, suggesting that it was not caused by the persistence of the strains in the area. However, turtle may be one of the main sources of outbreak of cholera outbreak in 2004.