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软刻蚀制备组织工程3D可生物降解、生物相容性好的几何图案聚合物微结构,方法简单,使用聚二甲基硅氧烷(PDMS)作为弹性铸膜.高分辨率透明掩膜图案用CorelDraw软件绘制并用高分辨率行式打印在塑料片上.制备微结构的聚合物材料为摩尔比85/15的聚乳酸/乙醇酸(PLGA)共聚物,对其进行预处理使之具有适当的硬度和柔韧性.用铸造、旋涂两种成模方法制备形体尺寸为10～100μm的PLGA肢手架.对影响肢手架结构完整及横向分辨率的因素进行了讨论和优化,并对两种成型方法的横向、纵向分辨率进行了比较.作为脚手架在组织工程上应用的概念验证,用热层压方法制造用于细胞培养的多层聚合物结构.实验结果表明,软刻蚀技术提供了一种实现组织工程微尺度3D聚合物结构的有效工具.
Soft-etching for the fabrication of 3D biodegradable, biocompatible microstructures of polymer microstructures with a simple method using polydimethylsiloxane (PDMS) as the elastic cast film High-resolution transparent mask pattern Was drawn with CorelDraw software and printed on plastic sheets in high resolution lines.The microstructured polymeric material was prepared as a polylactic / glycolic acid (PLGA) copolymer with a molar ratio of 85/15, pretreated to have a suitable Hardness and flexibility.The PLGA limbs with body size of 10 ~ 100μm were prepared by casting and spin-coating methods.The factors influencing the integrity and lateral resolution of the limbs structure were discussed and optimized, The horizontal and vertical resolution of the methods were compared.As a proof of concept for the application of scaffolding in tissue engineering, a multilayered polymer structure for cell culture was fabricated by thermal lamination.The experimental results showed that soft etching provided An effective tool for tissue engineering microscale 3D polymer structures.