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目的研究急性二氧化碳(CO2)中毒大鼠应用高压氧吸氧、高浓度和低浓度常压吸氧3种不同氧疗方式治疗对血清酶学和电解质的影响。方法健康雄性SD大鼠60只,随机分为正常对照组(A组)、CO2染毒组(B组)、染毒后高压氧治疗组[压力为2个大气压(ATA),氧浓度100%](C组)、染毒后高浓度常压吸氧治疗组(氧浓度50%)(D组)、染毒后低浓度常压吸氧治疗组(氧浓度33%)(E组)。给予吸入CO2气体制作急性CO2中毒大鼠模型,染毒后分别给予不同方式氧疗后,检测各组大鼠血清肌钙蛋白Ⅰ(CTNⅠ)、肌酸激酶(CK)、天冬氨酸转氨酶(AST)、血钾、血钠、血氯水平。结果 B、D、E组CTNⅠ、CK、AST水平高于A组(P<0.05);C组与A组比较CTNⅠ、CK、AST水平差异无统计学意义(P>0.05)。B、D和E组血钾水平高于A组(P<0.05);A与C组血钾水平比较差异无统计学意义(P>0.05)。B、D和E组血钠和血氯水平低于A组(P<0.05);C与A组比较血钠和血氯水平差异无统计学意义(P>0.05)。结论急性CO2中毒大鼠高压氧治疗对于改善血清酶学的升高和纠正电解质的紊乱与其他氧疗方式相比,有显著疗效。
Objective To study the effects of hyperbaric oxygen inhalation, high concentration and low concentration atmospheric oxygenation on serum enzymes and electrolytes in acute carbon dioxide (CO2) poisoning rats. Methods Sixty healthy male Sprague-Dawley rats were randomly divided into normal control group (group A), CO2 exposure group (group B), hyperbaric oxygen treatment group (pressure 2 ATA, oxygen concentration 100% (Group C). After exposure to high concentration of oxygen, the group with high concentration of oxygen (50% oxygen) (group D) and the group with low concentration of oxygen (group 33%) under low pressure after oxygen exposure (group E). The model of acute CO2 poisoning was established by inhalation of CO2 gas. After being exposed to different oxygen therapy, the levels of serum CTN Ⅰ, CK and aspartate aminotransferase (AST) AST), potassium, sodium, blood chlorine levels. Results The levels of CTNⅠ, CK and AST in group B, D and E were significantly higher than those in group A (P <0.05). There was no significant difference in CTN Ⅰ, CK and AST between group C and group A (P> 0.05). The levels of potassium in groups B, D and E were higher than those in group A (P <0.05). There was no significant difference in serum potassium between groups A and C (P> 0.05). The levels of serum sodium and blood chlorine in group B, D and E were lower than those in group A (P <0.05). There was no significant difference in serum sodium and blood chlorine between group C and A (P> 0.05). Conclusion Hyperbaric oxygen therapy in acute CO2 poisoning rats has a significant effect on improving serum enzymology and correcting electrolyte imbalance compared with other oxygen therapy methods.