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目的:通过对饥饿小鼠和饱食小鼠血糖及肝糖原测定,了解饥饿及饱食对血糖和肝糖原有影响。方法:取体重在25g以上的健康小鼠60只,随机分成两组:饥饿组30只,实验前严格禁食不禁水30h;饱食组30只:自由摄食,饮水。然后采用蒽酮显色法测定小鼠肝糖原,用Folin-吴法测定小鼠血糖。结果:饥饿与饱食进行比较小鼠血糖及肝糖原都表现为明显的降低或升高趋势(即饥饿小鼠血糖及肝糖原降低,饱食小鼠血糖及肝糖原升高),且P<0.05具有统计学意义。结论:饱食后肝糖原增加,饥饿肝糖原逐渐降低;饱食后血糖明显升高,饥饿则血糖明显降低。
OBJECTIVE: To understand the effects of starvation and satiety on blood glucose and glycogen by measuring blood glucose and glycogen in starved and fed mice. Methods: Sixty healthy mice weighing 25g or more were randomly divided into two groups: 30 in the starvation group, 30h in the strict fasting before the experiment, and 30 in the full-feeding group. Then, the liver glycogen of mice was determined by anthrone colorimetric method and the blood glucose of mice was measured by Folin-Wu method. Results: Compared with starvation and satiety, the blood glucose and glycogen in mice showed a significant decrease or increase trend (ie, decreased blood glucose and glycogen, increased blood glucose and glycogen in the starved mice) And P <0.05 was statistically significant. Conclusion: After full feeding, glycogen increased, while the glycogen in the starvation decreased gradually. The blood sugar increased significantly after fed, while the blood sugar decreased significantly in starvation.