作者用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离斯氏狸殖吸虫抗原,并通过转移电泳转至国产混合纤维素酯滤膜上,用特异单克隆抗体进行识别,检测识别的抗原区带的分子量。制备的三株单克隆抗体(A_6、D_(10)、E_9)共识别6条抗原区带,抗原的分子量分别为182、152、109、21、17.8和14.5kd。 The authors used sodium dodecyl sulfate - polyacrylamide gel electrophoresis separation of S. clavicei antigen and transferred to the domestic hybrid cellulose ester filter by transfer electrophoresis, using specific monoclonal antibodies to identify and detect the antigen identified The molecular weight of the zone. Three monoclonal antibodies (A_6, D_ (10), E_9) were used to identify six antigenic bands. The molecular weights of the antigens were 182, 152, 109, 21, 17.8 and 14.5 kd, respectively.