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从100条SRAP引物中筛选出12条具有多态性谱带的引物,对福建省闽清县17份甜橄榄种质资源进行SRAP分析。结果表明,12条引物共扩增得到72个位点,59个位点具有多态性,多态性比率为81.94%;17份甜橄榄种质资源的相似系数0.796~0.987;其中,羊屎与小个子种质相似系数最高,为0.987。相似系数在0.846处,17份种质资源可分为两大类,胜利1号、羊屎、黄田、小箬、自来圆、山源1号和小个子为第一大类,其他为第二大类;进一步区分,在相似系数0.870处又可分为四类,第一类包括胜利1号、羊屎、黄田、小箬、山源1号和小个子,第二类只有自来圆,第三类包括红脸、竹岐1号、长营、徳士,其他为第四类。
Twelve primers with polymorphic bands were screened from 100 SRAP primers and SRAP analysis was carried out on 17 sweet olive germplasm resources in Minqing County of Fujian Province. The results showed that 72 loci were amplified by 12 primers, 59 loci were polymorphic, the ratio of polymorphism was 81.94%. The similarity coefficient of 17 sweet olive germplasm resources was 0.796 ~ 0.987. Among them, The germplasm similarity coefficient with the small one is 0.987. Similar coefficient of 0.846, 17 germplasm resources can be divided into two categories, the victory on the 1st, sheep shit, Huangtian, Xiao Bian, Zi Yuan, Shan Yuan 1 and small for the first category, the other for the first Two categories; further differentiation, at the similarity coefficient of 0.870 can be divided into four categories, the first category includes victory 1, sheep shit, Huangtian, Xiaodi, Hill source 1 and small, only the second round , The third category includes red face, bamboo Qi 1, Chang Ying, DS, the other for the fourth category.