取成年大白鼠(体重150—200克)的食管、腺胃、小肠下段和大肠,分别固定于纯甲醇和10%福尔马林液中24小时。另取肠系膜制成铺片标本,以5 ml 注射器分别抽取10%福尔马林和纯甲醇,直接喷射于铺片标本上,固定10—15分钟。福尔马林固定之标本需用蒸镏水洗一次,铺片干后可直接染色,其余标本按常规包埋切片。切片厚度4— The adult rats (body weight 150-200 g) of the esophagus, glandular stomach, small intestine and the large intestine, were fixed in pure methanol and 10% formalin for 24 hours. Membranes made of mesentery from another specimens to 5 ml syringe were extracted 10% formalin and pure methanol, spray directly on the spread of specimens, fixed 10-15 minutes. Formalin fixed specimens need to be washed with distilled water once, after the film can be directly stained dry, the remaining specimens according to conventional embedded sections. Slice thickness 4-