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目的:为了探讨基质细胞衍生因子-1(stromal cell derived factor-1,SDF-1)及其受体CXCR4在急性白血病血清及脑脊液中的变化及临床意义。方法:用酶联免疫吸附双抗体夹心法(ELISA)测定45例急性白血病患者的血清及40例急性白血病患者的脑脊液中SDF-1,并分别与正常对照组比较。结果:1.ALL患者组、AML患者组及正常对照组血清的SDF-1水平分别为4255.46±2685.83,2405.10±905.24,2338.24±671.21(pg/ml),其中ALL组患者明显高于AML组及正常组,且差异具有统计学意义(P<0.05)。CNSL患者组及非CNSL患者组SDF-1血清水平分别为3696.30±432.49,3136.62±2157.64(pg/ml),组间无显著差别。2.ALL患者组、AML患者组及正常对照组脑脊液SDF-1水平分别为4576.15±1547.96,3097.20±1408.45,1983.61±857.98(pg/ml),其中急性白血病组高于正常对照组,差异具有统计学意义(P<0.05),ALL组高于AML组,差异也具有统计学意义(P<0.05)。CNSL患者组及非CNSL患者组脑脊液SDF-1水平分别为5236.30±1635.89,3640.07±1636.76(pg/ml),CNSL患者组高于非CNSL患者,差异具有统计学意义(P<0.05)。结论:脑脊液SDF-1水平的增高与CNSL的发生密切相关,有助于判断预后。外周血SDF-1表达水平与CNSL无明显相关,CNSL可能更取决于局部SDF-1的水平。
Objective: To investigate the changes and clinical significance of stromal cell derived factor-1 (SDF-1) and its receptor CXCR4 in serum and cerebrospinal fluid of patients with acute leukemia. Methods: SDF-1 in cerebrospinal fluid of 45 patients with acute leukemia and 40 patients with acute leukemia was detected by enzyme-linked immunosorbent assay (ELISA) and compared with normal control group respectively. The levels of SDF-1 in patients withALL, AML and normal control group were 4255.46 ± 2685.83,2405.10 ± 905.24,2338.24 ± 671.21 (pg / ml), respectively, in ALL group was significantly higher than that in AML group and Normal group, and the difference was statistically significant (P <0.05). The serum levels of SDF-1 in CNSL patients and non-CNSL patients were 3696.30 ± 432.49 and 3166.62 ± 2157.64 (pg / ml), respectively, with no significant difference between the two groups. The levels of SDF-1 in ALL patients, AML patients and normal controls were 4576.15 ± 1547.96,3097.20 ± 1408.45 and 1983.61 ± 857.98 (pg / ml), respectively, which were higher in acute leukemia group than in normal control group Significance (P <0.05), ALL group was higher than AML group, the difference was also statistically significant (P <0.05). The levels of SDF-1 in CSF of CNSL patients and non-CNSL patients were 5236.30 ± 1635.89 and 3640.07 ± 1636.76 (pg / ml), respectively, which were significantly higher in CNSL patients than in non-CNSL patients (P <0.05). Conclusion: The increase of SDF-1 in cerebrospinal fluid is closely related to the occurrence of CNSL, which is helpful to judge the prognosis. The level of SDF-1 in peripheral blood was not significantly correlated with CNSL, and CNSL may be more dependent on the level of local SDF-1.