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利用大麦第５染色体组上１９个ＳＴＳ－ＰＣＲ引物对中国春小麦（ＣＳ）及其突变体ｐｈ１ｂ基因组总ＤＮＡ进行ＰＣＲ扩增，结果发现有一对引物能有效地鉴定Ｐｈ１基因的缺失片段，其扩增特异片段大小约９２０ｂｐ，经中国春第５组同源群缺体－四体及５ＢＬ染色体双端体证明，该特异片段位于５Ｂ染色体长臂上。利用这个特异引物可以有效、方便、快速地鉴定ＣＳ×ｐｈ１ｂ组合的Ｆ２群体及ＢＣ１（Ｆ１×ｐｈ１ｂ）群体中的ｐｈ１ｂｐｈ１ｂ基因型，并从分子水平上初步证明ｐｈ１ｂ基因可能呈简单的孟德尔式遗传。
A total of 19 STS-PCR primers were used to amplify the total DNA of Chinese spring wheat (phi) and its ph1b mutant from barley chromosome 5 genome. The results showed that a pair of primers could effectively identify the deletion fragment of Ph1 gene and amplify The specific fragment was about 920bp in length, which was confirmed by the exon 4 of the Chinese Spring Group 5 and the diploid of chromosome 5BL. The specific fragment was located on the long arm of chromosome 5B. This specific primer can be used to identify the ph1bph1b genotype of F2 population of CS × ph1b and BC1 (F1 × ph1b) population efficiently, conveniently and rapidly. It is also possible to prove ph1b gene may be a simple Mendelian gene at the molecular level .