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目的观察乙酰胆碱(ACh)对大鼠海马齿状回颗粒细胞下层成年神经发生的作用和影响。方法通过立体定位经侧脑室药物注射建立试验动物模型,实验组给予ACh,假手术组给予生理盐水,对照组只麻醉动物,不做任何手术处理,术后2h经腹腔给予核苷酸类似物BrdU。4周后处死动物,采用免疫组化的方法观察并计数海马齿状回颗粒细胞下层新生神经元。结果实验组海马齿状回BrdU+和BrdU+/CaBP+双标的细胞数目为637.00±39.50、491.00±47.29/hippocampi(N=3,n=36),而假手术组和对照组分别为339.00±17.62、305.00±17.62/hippocampi(N=3,n=36)和336.00±49.05、304.00±30.44/hippocampi(N=3,n=36),实验组分别与假手术组和对照组比较都有统计学差异(P<0.05),假手术组与对照组之间比较无统计学差异(P>0.05)。结论乙酰胆碱可以增加海马齿状回颗粒细胞下层新生神经元的数目,由此可能促进学习和记忆的形成。
Objective To investigate the effect of acetylcholine (ACh) on adult neurons in the lower granular layer of dentate gyrus in rats. Methods The experimental animal model was established by stereotactic injection through the lateral ventricle. The experimental group was given ACh, the sham-operated group was given normal saline, the control group was only anesthetized with no operation. The nucleotide analogue BrdU . After 4 weeks, the animals were sacrificed and immunohistochemistry was used to observe and count the neonate neurons of the granulosa cells in the dentate gyrus of the hippocampus. Results The numbers of BrdU + and BrdU + / CaBP + double labeled cells were 637.00 ± 39.50 and 491.00 ± 47.29 / hippocampi (N = 3, n = 36) in the dentate gyrus of experimental group and 339.00 ± 17.62 and 305.00 ± 17.62 / hippocampi (n = 3, n = 36) and 336.00 ± 49.05,304.00 ± 30.44 / hippocampi (n = 3, n = 36). There were significant differences between the experimental group and the sham-operated group and the control group P <0.05). There was no significant difference between sham operation group and control group (P> 0.05). Conclusion Acetylcholine can increase the number of newborn neurons in the granular layer of dentate gyrus of hippocampus, which may promote the formation of learning and memory.