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目的检测肝细胞癌（ＨＣＣ）患者术前血清基质金属蛋白酶－２（ＭＭＰ－２）水平以确定其与ＨＣＣ根治性切除术后转移复发的关系。方法采用 Ｃｏｘ风险分析模型对影响预后的；临床指标进行分析以确定影响 ＨＣＣ切除术后生存时间的临床指标。同时，应用 ＥＬＩＳＡ方法检测 ＨＣＣ患者术前血清 ＭＭＰ－２水平及 ｎｏｒｔｈｅｒｎ ｂｏｌｔ方法对２４例相应肿瘤组织ＭＭＰ－２ ｍＲＮＡ表达水平进行定量分析。结果 Ｃｏｘ风险分析模型显示肿瘤大小及肿瘤是否存在肝内播散是影响 ＨＣＣ切除术后生存时间的显著因素（Ｐ＝ ０．０２２和 Ｐ＝ ０．０４０），并以此划分为高和低转移复发倾向组。根治性切除术后高转移复发倾向组ＨＣＣ患者术前血清ＭＭＰ－２显著高于根治切除术后低转移复发倾向组［（２６．３９ ± ２．６４）ｎｇ／ｍｌ对（２４．８６ ±１．９５）ｎｇ／ｍｌ，Ｐ＜０．０５］。非根治性切除组ＨＣＣ患者术前血清ＭＭＰ－２水平也显著高于根治性切除组 ＨＣＣ患者， （２．４３ ± ３．１２） ｎｇ／ｍｌ对（２５．７２ ± ２．４５） ｎｇ／ｍｌ， Ｐ＜ ０．０１。 Ｎｏｒｔｈｅｒｎｂｌｏｔ显示， ＨＣＣ患者血清ＭＭＰ－２水平变化与其对应肿瘤组织ＭＭＰ－２ ｍＲＮＡ表达变化相一致。
Objective To detect the serum level of matrix metalloproteinase-2 (MMP-2) in patients with hepatocellular carcinoma (HCC) before its radical resection and recurrence after radical resection of HCC. Methods The Cox risk analysis model was used to analyze the prognostic and clinical indicators to determine the clinical indicators affecting the survival time after HCC resection. At the same time, ELISA was used to detect preoperative serum MMP-2 levels in HCC patients and the northern bolt method to quantitatively analyze MMP-2 mRNA expression in 24 corresponding tumor tissues. Results The Cox risk analysis model showed that tumor size and presence of intrahepatic spread were significant factors affecting survival time after HCC resection (P = 0.022 and P = 0.040), and were divided into high and low metastases. Relapse tendency group. Preoperative serum MMP-2 in HCC patients with high metastasis and recurrence after radical resection was significantly higher than that after radical resection[(26.39 ± 2.64) ng/ml vs. 24.86 ± 1] .95) ng/ml, P<0.05]. Preoperative serum MMP-2 levels were also significantly higher in HCC patients with non-radical resection than those with radical resection in HCC (2.43 ± 3.12) ng/ml vs. (25.72 ± 2.45) ng/ml , P< 0.01. Northernblot showed that the change of serum MMP-2 level in HCC patients was consistent with the change of corresponding MMP-2 mRNA expression in tumor tissues.