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目的 :观察养精种玉汤加减方剂的醇提取液对离体培养卵巢颗粒细胞孕酮生成的影响 ,并进行机制探讨 ;方法 :应用放免检测孕酮血清浓度 ;结果 :该提取液可增加颗粒细胞孕酮基础分泌量和hCG诱导下的增加分泌量 ,明显增加细胞cAMP的浓度。
OBJECTIVE: To observe the effect of alcohol extracts of Yangjing Zhenyu Decoction on the production of progesterone in cultured ovarian granulosa cells, and to explore the mechanism. Methods: To detect progesterone serum concentration by radioimmunoassay; Results: The extract can increase granules. Cell progesterone basal secretion and hCG induced increased secretion, significantly increased cell cAMP concentration.