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目的:了解知母、连翘等几种中药炮制品或提取物的抑菌活性,明确抑菌有效部位,对比分析知母炮制前后抑菌活性变化,探讨其炮制原理。方法:应用纸片法,对比观察知母炮制品与连翘等中药提取物的抑菌作用。结果:连翘籽挥发油作用于金黄色葡萄球菌与大肠杆菌,其抑制效果显著,且抑制作用随着浓度的升高而呈增长趋势;而锦灯笼宿萼醇提物作用于大肠杆菌抑制效果不明显,抑制金黄色葡萄球菌作用显著,当醇提取物浓度为90%时,作用最好;知母炮制前后抑菌环不明显。结论:锦灯笼宿萼醇提物与连翘籽挥发油抑制金黄色葡萄球菌、大肠杆菌作用显著。
OBJECTIVE: To know the antibacterial activity of several kinds of Chinese medicine preparations or extracts such as Anemarrhena asphodeloides, Forsythia suspense, and so on, and to find out the effective sites of antibacterial activity. Methods: The paper method was used to compare the antibacterial effect of traditional Chinese medicine extracts such as Zhimu products and Forsythia suspense. Results: The volatile oil of Forsythia suspensa was applied to Staphylococcus aureus and Escherichia coli, and its inhibitory effect was remarkable. The inhibitory effect increased with the increase of concentration. However, Obviously, the inhibition of Staphylococcus aureus significant effect, when the alcohol extract concentration of 90%, the best; before and after the Zhimu antibacterial ring was not obvious. CONCLUSION: Alcoholic extract of C. lanuginosa and F. forsythia volatile oil inhibit Staphylococcus aureus and Escherichia coli significantly.