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目的 :探讨nm2 3-H1基因在膀胱癌中突变及表达的意义。方法 :应用半定量逆转录聚合酶链反应 (RT-PCR)和银染单链构象多态性 (SSCP)方法检测nm2 3-H1基因在 2 5例膀胱癌组织及 15例对照组织中突变和表达情况。结果 :对照粘膜中未检测出nm2 3-H1基因突变 ,而在 2 5例膀胱癌组织中发现 6例出现PCR产物单链泳动状态异常 ,异常率为 2 4%。癌组织和对照组织均有nm2 3-H1基因mRNA的表达 ,88% (2 2 / 2 5 )的癌组织中nm2 3-H1mRNA高表达 ,但在不同分期、分级中未见统计学差异。随着肿瘤恶性度的增加 ,nm2 3-H1基因突变率和基因相对表达量的增加有协同性。结论 :提示nm2 3-H1基因高表达可能与膀胱肿瘤恶性表型的出现及肿瘤恶性进展有关 ;基因突变可能是癌组织中基因产物表达增高的原因之一。
Objective: To investigate the significance of mutation and expression of nm23-H1 gene in bladder cancer. Methods: The mutations of nm23-H1 gene in 25 cases of bladder cancer and 15 cases of control tissues were detected by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and silver staining single strand conformation polymorphism (SSCP) Express the situation. RESULTS: No mutation of nm23 3-H1 gene was detected in the control mucosa. However, in 25 cases of bladder cancer, 6 cases were found to have single-stranded electrophoresis of PCR products. The abnormal rate was 24%. The expression of nm23-H1 mRNA was detected in both cancer and control tissues, while the expression of nm23 3-H1 was higher in 88% (2 of 2/5) of cancerous tissues, but there was no statistical difference in different stages and grading. With the increase of malignancy, the mutation rate of nm23-H1 gene and the relative increase of gene expression are synergistic. Conclusion: The high expression of nm23-H1 gene may be related to the appearance of malignant phenotype of bladder tumor and the malignant progression of the tumor. Gene mutation may be one of the reasons for the increased expression of gene products in cancer tissues.