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目的 :探讨大黄对大鼠胃排空运动的影响及其机制 ,为临床应用大黄提供理论依据。方法 :32只 Wistar大鼠随机分为大黄组及对照组 ,分别给大鼠灌服大黄水提液或蒸馏水 1、6 h后 ,以葡聚糖蓝 - 2 0 0 0为胃肠内标记物观察大鼠胃排空的变化 ,同时用免疫组化染色观察胃窦肌间神经丛 P物质 (SP)、血管活性肠肽 (VIP)的分布变化。结果 :灌服大黄水提液后 ,大鼠胃排空运动显著抑制 (P <0 .0 5或 <0 .0 1) ,胃窦肌间神经丛 SP的分布明显减少 (P<0 .0 5或 <0 .0 1) ,VIP分布明显增加 (P <0 .0 5或 <0 .0 1)。结论 :大黄对大鼠胃运动有明显的抑制作用 ,其机制可能与胃窦肌间神经丛 SP的分布减少及 VIP的分布增加有关
Objective: To explore the effect of rhubarb on gastric emptying in rats and its mechanism, so as to provide a theoretical basis for clinical application of rhubarb. Methods: Thirty-two Wistar rats were randomly divided into two groups: rhubarb group and control group. Rats were given gavage water or distilled water for 1, 6 h, and dextran blue-200 was used as an intragastric marker. The change of rat gastric emptying was observed. At the same time, the distribution of substance P (SP) and vasoactive intestinal peptide (VIP) in the antrum myenteric plexus was observed by immunohistochemical staining. RESULTS: After emptying rhubarb aqueous extract, rats’ gastric emptying was significantly inhibited (P <0.05 or <0.01), and the distribution of SP in myenteric plexus of gastric antrum was significantly reduced (P <0. 5 or <0. 0 1), VIP distribution increased significantly (P <0. 0 5 or <0. 0 1). Conclusion : Rhubarb has a significant inhibitory effect on gastric motility in rats, and its mechanism may be related to the decrease in the distribution of SP in the antrum myenteric plexus and the increase in the distribution of VIP.