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目的:建立通窍耳聋丸的质量控制方法。方法:用TLC法对当归进行定性鉴别;HPLC法测定通窍耳聋丸中橙皮苷的含量,色谱柱为Diamonsil C_(18),柱温:35℃,流动相为甲醇-水(40∶60),检测波长:283 nm。结果:当归的定性鉴别专属性强。橙皮苷在0.234 2～1.561 2 mg·ml~(-1)范围内呈良好的线性关系,r=0.999 2,平均回收率为99.5%,RSD=1.1%(n=9)。结论:该方法简单、快速、准确、重复性好,专属性强。
Objective: To establish the quality control method of Tongqiao deafness pill. Methods: The content of hesperidin in Tongqiao deafness pill was determined by TLC. Diamonsil C_ (18) was used as the chromatographic column. The column temperature was 35 ℃ and the mobile phase was methanol-water (40:60) , Detection wavelength: 283 nm. Results: Angelica qualitative identification of strong specificity. Hesperidin had a good linear relationship in the range of 0.234 2 ~ 1.561 2 mg · ml ~ (-1), r = 0.999 2, with an average recovery of 99.5% and RSD = 1.1% (n = 9). Conclusion: The method is simple, rapid, accurate, reproducible and specific.