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目的:探讨脆性组氨酸三联体(FHIT)基因蛋白Fhit在喉咽癌组织中的表达状况及与该肿瘤生物学行为的可能关系。方法:采用兔抗人Fhit抗体与枸橼酸-微波-SP免疫组织化学方法检测66例喉咽癌标本中Fhit的表达状况,并分析其与组织学分级、淋巴结转移的关系。结果:①喉咽癌组织Fhit表达阳性率为42.42%(28/66),正常喉咽组织为83.33%(55/66),二者比较,P<0.01;②Fhit在高、中、低分化鳞状细胞癌中的表达阳性率分别为65.38%(17/26)、38.10%(8/21)和26.32%(5/19),各个病理分级之间比较,P<0.01;③无淋巴结转移组Fhit表达阳性率为75.00%(12/16),淋巴结转移组为36.00%(18/50),P<0.01。结论:喉咽癌Fhit的表达较正常组织降低,其表达状况与病理分级及淋巴结转移明显相关,提示Fhit表达降低可能对喉咽癌的演化和进展具有重要作用,并有可能成为一个新的预后指标。
Objective: To investigate the expression of Fhit gene Fhit in hypopharyngeal carcinoma and its possible relationship with the biological behavior of the tumor. Methods: The expression of Fhit in 66 specimens of hypopharyngeal carcinoma was detected by rabbit anti-human Fhit antibody and citric acid-microwave-SP immunohistochemistry. The relationship between Fhit expression and histological grade and lymph node metastasis was analyzed. Results: ① The positive rate of Fhit expression in hypopharyngeal carcinoma was 42.42% (28/66) and that in normal laryngeal pharyngeal tissues was 83.33% (55/66), P <0.01. (2) The positive rates of expression in squamous cell carcinoma were 65.38% (17/26), 38.10% (8/21) and 26.32% (5/19), respectively, and there was no significant difference between the pathological grades (P <0.01) Fhit positive rate was 75.00% (12/16), lymph node metastasis was 36.00% (18/50), P <0.01. Conclusion: The expression of Fhit in hypopharyngeal carcinoma is lower than that in normal tissues. The expression of Fhit is correlated with pathological grade and lymph node metastasis. It suggests that the decrease of Fhit expression may play an important role in the development and progression of hypopharyngeal carcinoma and may be a new prognostic factor index.