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利用“Y”型嗅觉仪测定稻虱缨小蜂对吡虫啉处理的水稻植株挥发物的行为反应,并通过GC-MS对挥发物组分进行了定性和定量分析。行为测试结果表明:吡虫啉和施用了吡虫啉的不同水稻品种挥发物对稻虱缨小蜂的寄主选择行为无明显影响。稻虱缨小蜂在不同浓度吡虫啉处理的水稻植株之间表现出明显的嗅觉偏好。当褐飞虱密度为10头/苗时,与高浓度吡虫啉处理相比,低浓度吡虫啉处理植株释放的挥发物引诱到了更多的稻虱缨小蜂(施药5天除外)。GC-MS分析发现:与对照植株释放的挥发物相比,吡虫啉处理的稻株挥发物组分种类不变;吡虫啉浓度不同导致水杨酸甲酯等8种组分的相对含量明显不同;同时一些组分的相对含量因吡虫啉浓度与水稻品种、褐飞虱密度及施药天数等因子的互作发生了明显改变。
The response of imidacloprid-treated rice plant volatiles was determined using the “Y” -type olfactometer and qualitative and quantitative analysis of volatile components by GC-MS. The results of behavioral tests showed that the volatiles of imidacloprid and imidacloprid-treated rice had no significant effect on the host-selection behavior of B. tabaci. The testis showed significant olfactory preference between different concentrations of imidacloprid-treated rice plants. When the N. lugens density was 10 heads / seedling, the volatiles released by low concentrations of imidacloprid-treated plants induced more Myrmetoides (except for 5 days of application) compared to the high-concentration imidacloprid treatment. GC-MS analysis showed that the volatile components of imidacloprid-treated rice plants did not change when compared with the volatiles released by the control plants. The different imidacloprid concentrations resulted in significant differences in the relative contents of eight components such as methyl salicylate. At the same time, The relative content of some components changed significantly due to the interaction of imidacloprid concentration with rice varieties, the density of brown planthopper and the number of application days.