对白绢病菌（ＳｃｌｅｒｏｔｉｕｍｒｏｌｆｓｉＳａｃｃ）菌核的培养基种类、培养基量、培养方法等进行了研究，结果表明，以带皮的马铃薯配制的琼脂培养基培养菌核比传统的ＰＤＡ培养基的培养效果好；培养基最适使用量，以每个直径８５ｍｍ培养皿使用２０ｍｌ为佳，培养所得菌核数量较多且培养菌核所需时间最短；培养方法以菌落长满培养皿时翻面这种方法能达到快速、经济、大量地培养菌核的目的 The results showed that the cultivation of sclerotinia sclerotiorum in Sclerotium rolfsii Sacc was better than that of the traditional PDA culture medium ; The optimum amount of medium used to each diameter of 85mm Petri dishes 20ml better, the number of culture sclerotia generated more and the culture of sclerotia required the shortest time; culture method with the colonization Petri dish upset when this method Can achieve the purpose of culturing sclerotia rapidly, economically and in large quantities