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B-50(GAP-43)蛋白是一种神经生长相关蛋白,对于神经发育、外周神经损伤后再生及突触可塑性等过程有重要意义。本组曾报道损伤大鼠尾壳核后B-50 mRNA呈高表达。为探讨生理条件下经过学习记忆练习后与学习记忆相关脑区中B-50 mRNA的表达是否有变化,本实验将雌性成年Wistar大鼠分为实验组、假训组和安静对照组,实验组和假训组训练前禁水,按本组建立的方法,于Y迷宫中对实验组进行饮水奖励空间变换作业训练,假训组亦放入相同的迷宫内任其自由活动,但禁水。每日训练结束后只给动物自由饮水半小时。对照组不禁水亦不置入迷宫内。当实验组的正确反应率达80%以上并保持1周时,将3组动物同时断头
B-50 (GAP-43) protein is a nerve growth-related protein that is important for neural development, regeneration after injury of peripheral nerves and synaptic plasticity. This group has been reported after injury of caudate putamen nuclear B-50 mRNA was highly expressed. In order to investigate whether there is any change in the expression of B-50 mRNA in learning and memory-related brain regions after learning and memory exercises under physiological conditions, female adult Wistar rats were divided into experimental group, pseudo-training group and quiet control group, experimental group And false training group before the training of water, according to the method established in this group, in the Y maze experimental group for drinking water reward space shift homework training, false training group also placed in the same maze within their free time, but no water. After the end of daily training, animals are given free access to water for half an hour. Control group can not help but into the water inside the maze. When the correct response rate of the experimental group reached more than 80% for 1 week, the 3 animals were simultaneously decapitated