以龙眼胚性愈伤组织诱导产生子叶形胚状体为试验材料，比较了蔗糖、活性炭的质量浓度，椰乳的体积分数，光照度和成熟时间等因素对体胚“成熟”的影响以及不同成熟过程、成熟时间、胚状体形态、品种（基因型）等因素对龙眼体细胞胚胎萌发的影响．结果表明，龙眼体胚在含５０ｇＬ－１蔗糖、体积分数为０．０５的椰乳、１００ｍｇＬ－１肌醇的ＭＳ固体培养基上，在黑暗或弱光条件下，不论白色还是透明化（玻璃化）子叶形小胚状体，都可完成“成熟”过程．体胚对成熟培养的蔗糖质量浓度和成熟时间有特定的要求．经过成熟培养的体细胞胚胎，可高频率萌发成苗 The embryogenic callus induced by embryogenic callus of longan was used as experimental material. The effects of sucrose, the mass concentration of activated carbon, the volume fraction of coconut milk, light intensity and maturation time on the “maturation” of somatic embryos and the effects of different mature Process, maturation time, embryoid morphology, variety (genotype) and other factors on somatic embryos germination. The results showed that the longan somatic embryos were cultured on MS solid medium containing 50g L-1 sucrose, coconut milk with a volume fraction of 0.05 and 100mg L-1 myo-inositol in dark or low light conditions, whether white or transparent ) Cotyledons small embryoid body, can be completed “mature” process. Somatic embryos have specific requirements for the sucrose concentration and maturation time of mature culture. After maturation of somatic embryos, germination can be high frequency germination