【摘 要】
:
As a class of powerful molecular tool,antisense oligonucleotides(ASOs)are not only broadly used in protein and RNA biology,but also a highly selective therapeutic strategy for many diseases.Although the concept that ASO reagents only reduce expression of
【机 构】
:
School of Life Sciences,Chongqing University,Chongqing 400044,China;Center of Plant Functional Genom
论文部分内容阅读
As a class of powerful molecular tool,antisense oligonucleotides(ASOs)are not only broadly used in protein and RNA biology,but also a highly selective therapeutic strategy for many diseases.Although the concept that ASO reagents only reduce expression of the targeted gene in a post-transcriptional manner has long been established,the effect and mechanism of ASO reagents on RNA polymerase Ⅱ(Pol Ⅱ)transcription are largely unknown.This raised question is particularly important for the appropriate use of ASOs and the valid interpretation of ASO-mediated experiments.In this study,our results show that linear RNA ASO attenuates transcription of nascent transcripts by inducing premature transcription termination which is combina-torially controlled by Integrator,exosome,and Rat1 in Drosophila.However,circular RNA(circRNA)ASO transfection does not affect transcription activity of the encoded gene.These data suggest that the ASO technique can be applied to study a circRNA-mediated but not linear RNA-mediated function for its encoded gene locus.
其他文献
Covalent organic framework(COF)materials have great development value in the biomedical field.However,the huge size and poor dispersion of COF materials severely limit their application.Here we successfully prepared COF nanoparticles with uniform size and
Owing to excellent conductivity and abundant surface terminals,MXene-based heterostructures have been intensively in-vestigated as energy storage materials.However,elaborate design of the structure and composition of MXene-based hybrids towards superior e
The re-emergence of Zika virus(ZIKV)and its associated neonatal microcephaly and Guillain-Barré syndrome have led the World Health Organization to declare a global health emergency.Until today,many related studies have successively reported the role of va
In small-molecule organic solar cells(SM-OSCs),it remains a big challenge to obtain favorable bulk heterojunction morphology by donor material design.Herein,we design and synthesize three small-molecule donors BPF3T-C4,BPF3T-C6 and BPF3T-C8,with different
The employment of an intrinsic quinoidal building block,benzodipyrrolidone(BDP),on constructing conjugated polymers(PBDP-2F and PBDP-2CN)with high electron mobility and unipolar transport characteristic in polyethylenimine ethoxylated(PEIE)modified organi
In line with the“ecosystem approach”of the Convention on Biological Diversity(CBD)(Secretariat of the Convention on Biological Diversity,2004)toward recognizing the ser-vice value in maintaining the essential balance of biodi-versity,there has been a conc
Dear Editor,rnHypertension was reportedly the most common coexisting condition of COVID-19 as 15%-31.2%patients with COVID-19 had hypertension,and the incidence of hy-pertension reached 58.3%in COVID-19 patients requiring ICU care(Wang et al.,2020).Howeve
Mood disorders,also known as affective disorders,result in a consistent disturbance in mood.There are two major sub-types of mood disorders,i.e.,major depressive disorder(MDD) and bipolar disorder (BD).Mood disorders affect hundreds of millions of people
Dear Editor,rnMicroRNAs(miRNAs)are endogenous small RNAs(sRNAs)that play crucial regulatory roles in gene expres-sion.In plants,the transcribed primary miRNAs(pri-miR-NAs)are sequentially processed by DICER-LIKE 1(DCL1)into precursor miRNAs(pre-miRNAs),an
Bacteria growth depends crucially on protein synthesis,which is limited by ribosome synthesis.Ribosomal RNA(rRNA)transcription is the rate-limiting step of ribosome synthesis.It is generally proposed that the transcriptional initiation rate of rRNA operon