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目的 观察大鼠放射诱导脑损伤后海马区IL - 6mRNA的动态表达 ,探讨其在放射性脑损伤急性期反应的发病机制中可能的作用。方法 制备大鼠脑放射性损伤模型。应用逆转录聚合酶链反应 (RT -PCR)半定量分析大鼠脑放射性损伤后海马区在不同时间、不同剂量水平IL - 6基因转录的动态表达。结果 正常组海马区IL - 6mRNA低水平表达 ;全脑照射后表达逐步上调并在 12h达峰值 ,是正常组的 2～ 5倍 (P <0 .0 5 ) ,15Gy组上升幅度最大 (P <0 .0 0 1) ,高出 2Gy组和 30Gy组 1倍多 (P <0 .0 5 ) ;30Gy组低于 2Gy组 ,但无显著差异 (P >0 .0 5 ) ;在 6h时间点也可见到这一趋势 ,15Gy组与 30Gy组仍有显著差异 (P <0 .0 5 )。各照射组在 2 4h后恢复到基础水平。 结论 大鼠放射性脑损伤后海马区IL - 6mRNA表达上调 ,参与了脑放射性损伤急性期的细胞反应。
Objective To observe the dynamic expression of IL - 6 mRNA in hippocampus after radiation - induced brain injury in rats and its possible role in the pathogenesis of acute brain injury induced by radioactive brain injury. Methods Rat brain models of radiation injury were prepared. Semi - quantitative RT - PCR was used to analyze the dynamic expression of IL - 6 gene transcription in hippocampus at different time and different doses after rat brain radiation injury. Results The expression of IL - 6 mRNA in the hippocampus of rats in normal control group was lower than that in control group. The expression of IL - 6 mRNA in hippocampus of normal control group was gradually increased and peaked at 12h, which was 2 ~ 5 times higher than that in normal control group (P <0.05). (P <0.05), while those in 30Gy group were lower than those in 2Gy group, but there was no significant difference (P> 0.05); at 6h time point This trend can also be seen, 15Gy group and 30Gy group still significant differences (P <0. 05). Each irradiation group returned to the basic level after 24 hours. Conclusion The up - regulation of IL - 6mRNA in hippocampus after radiation brain injury in rats is involved in the cellular response in acute phase of brain radiation injury.