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目的:观察益气活血复方对心肌梗死后慢性心衰(chronic heart failure,CHF)大鼠核呼吸因子-1(nuclear respiratory factor-1,NRF-1)、线粒体转录因子A(mitochondrial transcription factor A,mtTFA)mRNA及蛋白表达的影响。方法:选用SPF级健康雄性SD大鼠80只,随机选取20只为空白组,其余大鼠采用左冠状动脉前降支结扎法配合力竭式游泳及节食方法复制CHF大鼠模型。将造模成功的大鼠随机分为中药组、西药组及模型组。空白组与模型组给予常规等容积蒸馏水灌胃,每日2次,中药组灌胃益气活血复方[9.2 g生药/(kg·d)]日2次,西药组灌胃赖诺普利[1.5 mg生药/(kg·d)]日2次。持续给药28 d后,禁食不禁水12 h,取心肌组织采用实时荧光定量PCR和免疫蛋白印迹法检测NRF-1、mtTFA、mRNA及蛋白表达,ELISA法测定BNP浓度。结果:与空白组比较,治疗组及模型组BNP浓度升高(P<0.05)。与模型组比较,治疗组BNP浓度降低(P<0.05)。中药组与西药组比较,BNP浓度无差异(P>0.05)。与空白组比较,模型组NRF-1、mtTFA mRNA及蛋白表达降低(P<0.05)。与模型组比较,中药组和西药组NRF-1、mtTFA mRNA及蛋白表达升高(P<0.05)。中药组与西药组NRF-1、mtTFA mRNA及蛋白的表达比较,差异无统计学意义(P>0.05)。结论:益气活血复方可通过激活与心肌能量代谢有关的NRF-1、mtTFA通路,增加线粒体的产能,以此来改善和纠正CHF。
Objective: To observe the effects of Yiqi Huoxue Compound on nuclear respiratory factor-1 (NRF-1), mitochondrial transcription factor A (A) mtTFA) mRNA and protein expression. Methods: Eighty SPF healthy male Sprague-Dawley rats were randomly selected. Twenty rabbits were randomly selected as the blank group. Resting rats were sacrificed by the left anterior descending coronary artery ligature method combined with exhaustive swimming and dieting methods. The successful model rats were randomly divided into traditional Chinese medicine group, western medicine group and model group. The rats in the blank group and the model group were given conventional isotonic distilled water twice a day for 2 days. The Chinese medicine group was given gavage [9.2 g crude drug / (kg · d)] twice daily and the western medicine group was administered with lisinopril [ 1.5 mg crude drug / (kg · d)] 2 times a day. After continuous administration for 28 days, fasting for 12 h was performed. The myocardial tissue was taken for detection of NRF-1, mtTFA, mRNA and protein expression by real-time fluorescence quantitative PCR and Western blotting. BNP concentration was determined by ELISA. Results: Compared with the blank group, the concentration of BNP in treatment group and model group increased (P <0.05). Compared with the model group, the treatment group BNP concentration decreased (P <0.05). There was no difference in BNP concentration between traditional Chinese medicine group and western medicine group (P> 0.05). Compared with the blank group, the expression of NRF-1 and mtTFA mRNA and protein in model group decreased (P <0.05). Compared with the model group, the expressions of NRF-1 and mtTFA mRNA and protein were increased in the traditional Chinese medicine group and the western medicine group (P <0.05). The expression of NRF-1, mtTFA mRNA and protein in TCM group and western medicine group had no significant difference (P> 0.05). Conclusion: Yiqi Huoxue Compound can improve and correct CHF by activating NRF-1, mtTFA pathway related to myocardial energy metabolism and increasing mitochondrial productivity.