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目的探讨白芍总苷(TGP)对人永生化角质形成细胞(HaCaT细胞)增生及分泌细胞间黏附分子(ICAM)-1的影响,探讨可能涉及的信号传导通路。方法用500 U/ml干扰素(IFN)-γ诱导HaCaT细胞,不同浓度TGP(0.5～312.5μg/ml)作用于HaCaT细胞。四甲基偶氮唑盐(MTT)法观察TGP对HaCaT细胞增生活性的影响。酶联免疫吸附试验(ELISA)及荧光免疫组化法检测HaCaT细胞表达ICAM-1的水平。结果 TGP在低浓度(0.5～25.0μg/ml)时对HaCaT细胞增生活性有促进作用,在高浓度(62.5～125.0μg/ml)时对HaCaT细胞增生活性呈抑制作用。0.5～25.0μg/ml TGP可显著降低HaCaT细胞表达ICAM-1的水平(P<0.05,P<0.01)。结论 TGP对IFN-γ上调HaCaT细胞分泌ICAM-1有显著的抑制作用,可能是TGP抗炎作用机制之一。
Objective To investigate the effects of total glucosides of paeony (TGP) on proliferation and secretion of intercellular adhesion molecule - 1 (ICAM - 1) in human immortalized keratinocytes (HaCaT cells) and to explore the possible signaling pathways involved. Methods HaCaT cells were induced with 500 U / ml interferon (IFN) -γ, and treated with different concentrations of TGP (0.5-312.5 μg / ml) in HaCaT cells. The effect of TGP on the proliferation of HaCaT cells was observed by MTT assay. The expression of ICAM-1 in HaCaT cells was detected by enzyme-linked immunosorbent assay (ELISA) and fluorescence immunohistochemistry. Results TGP could promote the proliferation of HaCaT cells at low concentration (0.5 ~ 25.0μg / ml), and inhibit the proliferation of HaCaT cells at high concentration (62.5 ~ 125.0μg / ml). 0.5 ~ 25.0μg / ml TGP could significantly decrease the expression of ICAM-1 in HaCaT cells (P <0.05, P <0.01). Conclusion TGP can inhibit the secretion of ICAM-1 from HaCaT cells induced by IFN-γ, which may be one of the anti-inflammatory mechanisms of TGP.