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为了揭示病原真菌白僵菌Beauveria侵染昆虫过程中如何利用虫体内糖类物质作为自身营养,本研究测定了布氏白僵菌Beauveria brongniartii(Sacc.)Petch(2382菌株)感染油松毛虫Dendrolimus tabulaeformis Tsai et Liu幼虫后,虫体血淋巴中酸性海藻糖降解酶活性及海藻糖和葡萄糖含量的变化。油松毛虫4龄幼虫感染菌株孢子悬浮液后,血淋巴中酸性海藻糖降解酶的活性明显高于对照组,感染后第3天酶活性达到最大值(0.2786 U/mg),此后第4-6天酶活性逐渐降低;染菌后的6 d中,血淋巴中海藻糖含量显著低于对照组,同样在感染后第4天其含量逐渐降低,第6天时降到最低值。相比之下,处理组血淋巴中的葡萄糖含量显著高于对照组;处理组其含量在第1-3天内呈现快速升高趋势,在第3天达到最大值(7.7615 mmol/L),然后逐渐降低。结果说明,白僵菌侵入昆虫血淋巴后,菌株代谢产生酸性海藻糖降解酶,将血淋巴中的海藻糖水解成为葡萄糖,然后为真菌利用,破坏了虫体内的血糖平衡,这是一个相互连接的生理代谢和生化反应过程。
In order to reveal how Beauveria Beauveria infects insects using the saccharide of the parasites as their own nutrition, Beauveria brongniartii (Sacc.) Petch strain 2382 was infected with Dendrolimus tabulaeformis Changes of Acid Trehalose Degrading Enzyme Activity and Trehalose and Glucose Contents in the Hemolymph of Tsai et Liu Larvae. The 4th instar larvae of Pinus trituberculatus infected with the spore suspension, the activity of acid trehalose degrading enzyme in the haemolymph was significantly higher than that of the control group, and reached the maximum (0.2786 U / mg) on the 3rd day after infection, On the 6th day after infection, the content of trehalose in the hemolymph was significantly lower than that in the control group. The content of trehalose in the hemolymph gradually decreased on the 4th day after infection and dropped to its lowest value on the 6th day. In contrast, the content of glucose in the hemolymph of the treatment group was significantly higher than that of the control group; the content of the treatment group showed a rapid increase trend in the first 1-3 days and reached the maximum value of 7.7615 mmol / L on the third day Gradually decreases. The results showed that Beauveria bassiana invade the hemolymph of insects, the strains produce acidic trehalose-degrading enzyme metabolism, the hemolymph trehalose hydrolysis into glucose, and then used for fungi, destruction of the body blood glucose balance, which is a mutual connection The physiological metabolism and biochemical reaction process.