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采用淹水非种植水稻土微环境模式系统,对水稻土进行1h和1、5、10、20、30d淹水培养,利用PCR-DGGE技术检测、多元统计分析淹水培养过程中地杆菌科微生物的群落结构和多样性变化规律及其影响因子。结果表明,淹水培养过程中地杆菌科微生物群落结构发生了明显的演替性变化:由r-对策优势种群演替至k-对策优势种群,且群落结构由不稳定向稳定演变(培养1h和1d处理间相似性最低,群落结构变化最大,20d和30d处理间相似性最高,群落结构变化最小);该过程中,地杆菌科微生物物种丰富度指数和Shannon-Weiner指数在1h处理中均为最小,5d处理的最大;CCA相关性分析表明,淹水培养过程中Fe(Ⅱ)浓度与群落结构多样性指数呈正相关,证实该类微生物对于异化铁还原能力具有重要作用;测序结果表明,19个优势DGGE条带与来自水稻土中的未培养地杆菌科微生物亲缘关系相近。
The paddy soil was flooded at 1h, 1, 5, 10, 20 and 30 days by using the micro-environmental model system of flooded and non-planted paddy soils. PCR-DGGE was used to detect paddy soil. The community structure and diversity of the law of variation and its impact factors. The results showed that there was a significant succession of microbial communities in the Geobacter family during the flooding process: from dominant species of r-strategy to dominant species of k-strategy, and the community structure changed from unstable to stable And 1d treatment, the community structure changed the most, the similarity between the 20d and 30d treatments was the highest, and the community structure changed the least.) In the process, the species richness index and Shannon-Weiner index CCA correlation analysis showed that there was a positive correlation between Fe (Ⅱ) concentration and diversity index of community structure in submerged culture, which confirmed that this kind of micro-organism had an important effect on the iron reduction ability of iron. The results of sequencing showed that, Nineteen dominant DGGE bands were closely related to uncultivated Geobacteriaceae from paddy soil.