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目的:探讨槟榔对大鼠胃肠运动的影响及其机制,为临床应用槟榔提供理论依据。方法:48只Wistar大鼠随机分为低浓度(25%)槟榔组(A组)、高浓度(100%)槟榔组(B组)、对照组(蒸馏水)(C组),分别给大鼠灌服低、高浓度槟榔液、蒸馏水1、6h后,以葡聚糖蓝-2000为胃肠内标记物,观察大鼠胃肠动力变化;同时用放射免疫分析法测定血浆胃动素(MTL)、P物质(SP)、血管活性肠肽(VIP)的分布变化。结果:灌服低、高浓度槟榔液1、6h后大鼠胃排空运动明显增加(P<0.01或P<0.05),血浆胃动素、P物质分布明显增加(P<0.01或P<0.05),血管活性肠肽分布明显减少(P<0.01或P<0.05)。结论:槟榔对大鼠胃运动有明显促进作用,其机制可能与血浆P物质、胃动素分布增加及血管活性肠肽分布减少有关。
Objective: To investigate the effects of betel nut on gastrointestinal motility in rats and its mechanism, so as to provide theoretical basis for the clinical application of betel nut. Methods: Forty-eight Wistar rats were randomly divided into low concentration (25%) betel nut group (group A), high concentration (100%) betel nut group (group B), and control group (distilled water) (group C). After low and high concentrations of betel nut solution and distilled water for 1 and 6 h, dextran blue-2000 was used as a gastro-intestinal marker to observe the changes of gastrointestinal motility in rats; simultaneously, plasma motilin was measured by radioimmunoassay (MTL). Changes in the distribution of substance P (SP) and vasoactive intestinal peptide (VIP). RESULTS: Gastric emptying was significantly increased in rats fed with low and high concentrations of betel nut solution for 1 and 6 h (P<0.01 or P<0.05), and the distribution of plasma motilin and substance P was significantly increased (P<0.01 or P<0.05). The distribution of vasoactive intestinal peptide was significantly reduced (P<0.01 or P<0.05). Conclusion: The betel nut has obvious promoting effect on the gastric motility in rats. The mechanism may be related to the increase of the distribution of plasma substance P, motilin and the decrease of vasoactive intestinal peptide.