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关于癌组织的代谢及酶谱,国内外学者进行过许多研究,它们大多集中于动物的实验肿瘤,对人体的肿瘤尤其是胃癌的酶谱研究较少。Goldman(1964)曾广泛研究过人体各种正常组织、各部肿瘤(包括胃癌)的乳酸脱氢酶(LDH)活性,并认为胃癌组织的LDH活性与正常胃粘膜相近,但其同功酶则M型明显突出,Piper(1965)发现胃癌组织的LDH活力比幽门粘膜的LDH活力为高,但与胃体粘膜相比差异不明显;然而他们发现胃癌组织的谷草转氨酶(GOT)活力比正常胃粘膜的为低;Tsunematsu(1969)发现胃癌组织中醛缩酶(ALD)同功酶明显增高;赤松昌亮(1970)用组化方法发现胃癌组织的LDH活力增高,而葡萄糖6磷酸脱氢酶(G6PDH)呈中等活力。国内文献尚未发现有关资料。本研究试图通过测定我国人体胃癌组织中的几种主要糖代谢的酶活力以及几种转移酶类及磷酸酶的活性,了解胃癌组织与正常组织间代谢及酶谱的差别,并为从胃液中测定这些酶类活力,以筛选对胃癌诊断有价值的酶类奠定基础。
With regard to the metabolism and zymogram of cancer tissues, many scholars at home and abroad have conducted many studies. Most of them focus on experimental tumors in animals. There are few studies on the zymogram of human tumors, especially gastric cancer. Goldman (1964) has extensively studied the activity of lactate dehydrogenase (LDH) in various normal tissues and tumors (including gastric cancer) of human body. It is considered that the LDH activity of gastric cancer tissue is similar to that of normal gastric mucosa, but its isozyme is M. The type is obviously prominent. Piper (1965) found that the LDH activity of gastric cancer tissue was higher than that of pylorus mucosa, but the difference was not significant compared with the gastric mucosa; however, they found that the gastric mucosal tissue had a lower activity of aspartate aminotransferase (GOT) than normal gastric mucosa. Tsunematsu (1969) found that the aldosterase (ALD) isoenzyme in gastric cancer tissue was significantly increased; Akamatsu (1970) found that the LDH activity of gastric cancer tissue was increased by histochemical method, while glucose-6-phosphate dehydrogenase (G6PDH) ) Is of medium vitality. Domestic literature has not yet found relevant information. This study sought to determine the differences in metabolism and zymograms between gastric cancer tissues and normal tissues by measuring the enzyme activities of several major glucose metabolism enzymes and the activities of several transferases and phosphatases in human gastric cancer tissues in China. The activity of these enzymes was determined to lay the foundation for the screening of enzymes that are valuable for the diagnosis of gastric cancer.