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分蘖角度是构成水稻理想株型和高产育种的重要农艺性状之一.通过对水稻散生突变体的遗传学分析认为,该散生表型受一隐性核基因控制,与已报道的水稻散生突变体la等位,故将此突变体命名为la-2,而原突变体被重新命名为la-1.利用la-2与w11和浙福802分别杂交产生的F2群体对LA位点进行遗传定位,发现其与第11号染色体上的微卫星标记RM202和RM229连锁,遗传距离分别为10.0和8.0 cM.通过进一步在两标记间发展的6个新的分子标记,将该基因精确地定位于约0.4 cM的区间.为进一步克隆LA基因和探讨水稻分蘖角度的控制机制奠定了良好的基础.
The tillering angle is one of the important agronomic traits that make up the ideal plant type and high-yielding breeding in rice.Through the genetic analysis of the intersitial rice mutant, it is considered that this plant phenotype is controlled by a recessive nuclear gene, The mutant was named as la-2, while the original mutant was renamed la-1.Furthermore, the F2 locus generated by crossing la-2 with w11 and Zhefu 802, respectively, The genetic mapping was found to be linked to the microsatellite markers RM202 and RM229 on chromosome 11 with a genetic distance of 10.0 and 8.0 cM respectively.6 The gene was further precisely amplified by 6 new molecular markers that developed between the two markers Which located in the interval of about 0.4 cM, which laid a good foundation for further cloning LA gene and exploring the control mechanism of rice tillering angle.