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为了解小麦NaN3诱变后代籽粒性状的遗传变异规律,利用高通量表型分析软件SmartGrain对小麦新品种陕农33NaN3诱变群体(M3)籽粒性状进行测量,并进行相关分析、通径分析和主成分分析。结果表明,M3代籽粒性状变异系数表现为千粒重>密度因子>表面积>长宽比>粒宽>圆度>周长>粒长,诱变群体籽粒性状均值除长宽比外均较陕农33不同程度下降。千粒重与籽粒表面积、周长、粒长、粒宽、圆度、密度因子均呈极显著正相关,与长宽比呈极显著负相关。经通径分析,7个籽粒性状对千粒重直接贡献表现为密度因子>粒宽>表面积>周长>长宽比>圆度>粒长,其中,密度因子、粒宽和表面积对千粒重有较大的正效应。主成分分析结果显示,2个主成分(籽粒大小和籽粒形状)累计贡献率达到94.10%,说明2个主成分已经覆盖诱变群体所有籽粒性状的主要变异信息。
In order to understand the genetic variation of grain traits in mutants of wheat NaN3, the grain traits of Shannong33NaN3 mutagenized population (M3) of wheat cultivar Shannong33 were measured using high-throughput phenotypic analysis software SmartGrain, and correlation analysis, path analysis Principal component analysis. The results showed that the coefficient of variation of grain traits in M3 showed the performance of 1000-grain weight> density factor> surface area> aspect ratio> grain width> roundness> circumference> grain length. Decreased to varying degrees. Grain weight and grain surface area, perimeter, grain length, grain width, roundness, density factor showed a very significant positive correlation, and the aspect ratio was extremely significant negative correlation. According to the path analysis, the direct contribution of seven grain traits to 1000-grain weight was as follows: density factor> grain width> surface area> circumference> aspect ratio> roundness> grain length. Among them, density factor, grain width and surface area had greater effects on 1000-grain weight Positive effect. The principal component analysis showed that the cumulative contribution rate of the two principal components (grain size and grain shape) reached 94.10%, indicating that the two principal components have covered the main variation information of all the grain traits of the mutagenized population.