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目的:观察福尔马林致炎性鼠中枢三叉神经尾侧亚核(Sp5C)c-fos和胶质原纤维酸性蛋白(glialfibrilary acidic protein GFAP)表达的动态变化。方法:选择体重200-250g健康SD大鼠,在左上唇皮下注射2.5%福尔马林50μl建立炎性痛模型。分别在注射后30,60,120,240,360min等时间点处死,每组6只。免疫荧光染色观察各组大鼠同侧Sp5C核c-fos和GFAP表达的情况。结果:在面部皮下注射福尔马林后,fos阳性(fos-immunoreactivity,Fos-IR)神经元在注射后30分钟即可观察到,120分钟达到峰值,到360分钟观察结束仍有较高的表达。GFAP-IR在福尔马林注射后30分钟即有较高表达,60分钟达峰值后下降,240分钟即恢复到正常水平,二者分布区域相同。结论:在福尔马林致炎性痛模型中Sp5C内神经元和星形胶质细胞共同参与中枢神经系统对炎性疼痛刺激的调节,星形胶质细胞可能对神经元的活动有调节作用。
Objective: To investigate the dynamic changes of c-fos and glial fibrillary acidic protein (GFAP) expression in the rat sub-trigeminal nucleus (Sp5C) induced by formalin. METHODS: Healthy SD rats weighing 200-250 g were selected, and inflammatory pain models were established by subcutaneous injection of 50μl of 2.5% formalin into the left upper lip. The rats were sacrificed at 30, 60, 120, 240 and 360min after injection respectively, 6 rats in each group. Immunofluorescence staining was used to observe the expression of c-fos and GFAP in the ipsilateral Sp5C nucleus in each group. RESULTS: Fos-immunoreactivity (Fos-IR) neurons were observed 30 minutes after injection in the face subcutaneously and peaked at 120 minutes after the subcutaneous injection of formalin expression. GFAP-IR was expressed 30 minutes after formalin injection, peaked at 60 minutes and then decreased at 240 minutes, which returned to normal levels. CONCLUSIONS: Sp5C neurons and astrocytes are involved in the regulation of inflammatory pain stimuli in the central nervous system in formalin-induced inflammatory pain models. Astrocytes may have regulatory effects on neuronal activity.