论文部分内容阅读
目的检测五种寡糖在胃癌原发灶和淋巴结转移灶中的表达情况，探讨糖蛋白与转移的关系。方法收集伴有淋巴结转移的胃癌50例，行免疫组化和亲和组化染色。染色强度的判断采用了既考虑肿瘤的异质性又能客观地进行分析的半定量计分系统。统计学方法采用SAS系统软件，作配对t检验及相关分析。结果三种组织学类型原发灶和转移灶均有5种寡糖的表达，Slex在三种组织学类型的转移灶中的表达均显著高于原发灶（P＜0．05），SA-GalNAc在印戒细胞癌和低分化腺癌中的表达明显高于原发灶。50例转移灶中，5种寡糖的表达均与原发灶呈正相关（P＜0．01），其中相关系数最大的是含唾液酸的寡糖SA-GalNAc和Slex。结论原发灶表达唾液酸化的Slex和SA－GaLNAc高的癌细胞群更易发生淋巴结转移，这两种寡糖可作为判断胃癌预后的指标之一。
Objective To detect the expression of five kinds of oligosaccharides in primary gastric cancer and lymph node metastasis, and to explore the relationship between glycoprotein and metastasis. Methods Fifty cases of gastric cancer with lymph node metastasis were collected and immunohistochemistry and affinity staining were performed. The determination of staining intensity employs a semi-quantitative scoring system that considers both the tumor heterogeneity and the objective analysis. Statistical methods used SAS system software, paired t test and correlation analysis. Results There were 5 kinds of oligosaccharides in the primary and metastatic lesions of the three histological types. The expression of Slex in the three histological types of metastases was significantly higher than that of the primary lesions (P<0.05). -The expression of GalNAc in signet-ring cell carcinoma and poorly differentiated adenocarcinoma was significantly higher than that of the primary foci. In 50 metastases, the expression of five oligosaccharides was positively correlated with the primary lesions (P<0.01). Among them, the sialic acid-containing oligosaccharides SA-GalNAc and Slex had the largest correlation coefficients. Conclusion Lymph node metastasis is more likely to occur in cancer cells with high salivary Slex and SA-GaLNAc expression in primary tumors. These two kinds of oligosaccharides can be used as one of the indicators for judging the prognosis of gastric cancer.