【摘 要】
：
Cryptorchidism is associated with infertility in adulthood.Early orchiopexy is suggested to reduce the risk.Information is lacking on the potential link between infant germ cell maturation and the risk of future infertility.The objective of the study was
【机 构】
：
Laboratory of Reproductive Biology, Copenhagen University Hospital Rigshospitalet, Copenhagen 2100,
论文部分内容阅读
Cryptorchidism is associated with infertility in adulthood.Early orchiopexy is suggested to reduce the risk.Information is lacking on the potential link between infant germ cell maturation and the risk of future infertility.The objective of the study was to evaluate age-related germ cell development in cryptorchidism.Immunostaining for markers of germ cell development (octamer binding transcription factor 3/4 [0CT3/4],placental alkaline phosphatase [PLAP],KIT proto-oncogene [C-KIT],podoplanin [D2-40],Lin-28 homolog A [LIN28],and G antigen 7 [GAGE-7]) was performed in testicular biopsies from 40 cryptorchid boys aged 4-35 months.Germ cell numbers and distributions were evaluated in cross sections of seminiferous tubules,with and without immunostaining.OCT3/4,D2-40,and LIN28 were generally expressed in the early stages of germ cell development,as shown by positive expression in germ cells in the central region of seminiferous tubules.In contrast,PLAP and GAGE-7 were expressed in both central and peripheral parts of the tubules in the early stages of development and expressed mainly in a peripheral position with advancing age.Germ cell maturation was delayed in this study population as compared with that observed in our previous study on germ cell markers in a healthy population.The number of GAGE-7-positive germ cells per tubular cross section obtained by immunostaining was significantly higher than that obtained by standard hematoxylin and eosin staining.Double immunostaining revealed heterogeneity in germ cell development in cryptorchid testes.These results shed light on the pathophysiology of germ cell development in boys with cryptorchidism.
其他文献
Complete intracorporeal urinary diversion (ICUD) with an orthotopic neobladder is an emerging procedure.The objective of this article was to assess retrospectively the urodynamic and continence results after robot-assisted radical cystectomy (RARC) and IC
In about half the cases of involuntary childlessness,a male infertility factor is involved.The PIWI-LIKE genes,a subclade of the Argonaute protein family,are involved in RNA silencing and transposon control in the germline.Knockout of murine Piwi-like 1 a
Multiple measurements of nocturnal penile tumescence and rigidity (NPTR) are widely accepted as a method to differentiate psychogenic erectile dysfunction (ED) from organic ED.However,direct evidence remains limited regarding the first-night effect on NPT
Antisperm antibodies (ASAs) are assumed to be a possible causative factor for male infertility,with ASAs detected in 5％-15％ of infertile men but in only 1％-2％ of fertile ones.It remains unclear whether ASAs have an adverse effect on the outcome of in vitr
Androgen insensitivity syndrome (AIS),an X-linked recessive genetic disorder of sex development, is caused by mutations in the androgen receptor (AR) gene, and is characterized by partial or complete inability of specific tissues to respond to androgens i
Testosterone deficiency is common in men with cardiovascular disease (CVD), and randomized placebo-controlled trials (RCTs) have reported beneficial effects of testosterone therapy on exercise-induced cardiac ischemia in chronic stable angina, functional
The clinical predictive factors for malignant testicular histology remain unclear because of the low prevalence.Therefore,the aim of this study was to investigate predictors of malignant histology for testicular masses and decide more testis-sparing surge
We summarized our experience in transurethral seminal vesiculoscopy (TSV) for recurrent hemospermia by introducing surgical techniques, intraoperative findings, and treatment outcomes. TSV was performed in 419 patients with an initial diagnosis of persist
During spermatogenesis,developing germ cells that lack the cellular ultrastructures of filopodia and lamellipodia generally found in migrating cells,such as macrophages and fibroblasts,rely on Sertoli cells to support their transport across the seminifero
Human papillomavirus (HPV) is the most common sexually transmitted virus in males and females worldwide; yet its impact upon male fertility remains unclear. The objective of this study was to evaluate the potential impact of HPV infection in semen on male