为了解凝固酶阴性葡萄球菌（ＣＮＳ）在新生儿的感染情况及耐药特点，应用细菌自动培养系统（Ｂａｃｔ／Ａｌｂｅｒｔ），对已检出的 ２０３株 ＣＮＳ的菌种分布和药敏试验进行了分析。结果：表皮葡萄球菌（ＥＳ）１１５株，占 ＣＮＳ的 ５６．６％，以血标本最高，ＥＳ对青霉素、红霉素和复方新诺明的敏感性分别为９．５％、１２．１％和２９．５％，对万古霉素的敏感性为１００％。提示；表皮葡萄球菌，腐生葡萄球菌已成为新生儿ＣＮＳ感染的主要致病菌，Ｂａｃｔ／Ａｌｂｅｒｔ系统方法灵敏，培养基营养丰富，检出时间缩短，在新生儿败血症的诊断中具有重要价值。 In order to understand the infection and drug resistance of coagulase-negative staphylococci (CNS) in neonates, the strain distribution and drug susceptibility test of 203 CNS strains were detected by automatic bacterial culture system (Bact / Albert) analysis. Results: Staphylococcus epidermidis (ES) 115 strains accounted for 56.6% of CNS, with the highest blood samples. The sensitivity of ES to penicillin, erythromycin and cotrimoxazole was 9.5%, 12.1% And 29.5%, the sensitivity to vancomycin is 100%. It is suggested that Staphylococcus epidermidis and Staphylococcus saprophytica have become the main pathogenic bacteria of neonatal CNS infection. The method of Bact / Albert system is sensitive, the medium is rich in nutrients and the detection time is shortened, which is of great value in the diagnosis of neonatal sepsis.