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AIM:Ets1 proto-oncogene is a transcription factor involvedin the activation of several genes of tumor invasion andmetastasis.We aimed to determine the relationship betweenthe extent and intensity of Ets1 expression and patients’clinicopathological factors in gastric carcinoma.METHODS:Immunohistochemical analysis was performedfor gastric tumor paraffin-embedded sections,followed byimage analysis.RESULTS:Ets1 was not expressed in the normal gastricepithelium and its surrounding cells.The percentage of Ets1expressing cells detected increased significantly in bothepithelial tumor and stromal cells from high T classification,lymph node metastasis positive,clinical advanced-stagegroups(P<0.05).The level of Ets1 staining in epithelial tumorcells also reflected the degree of cell differentiation.Thepercentage of epithelial and stromal cells expressing Ets1was significantly correlated with the presence of lymph nodemetastasis(P=0.014 and P=0.001 respectively).Ets1expression was not observed in tissue samples from patientswith benign gastric ulcers.CONCLUSION:Ets1 protein expression in epithelial tumorcells reflects the degree of differentiation,and the percentageof Ets1 positive tumor and stromal cells correlates with lymphnode metastasis.Thus Ets1 is a valuable marker of malignantpotential in terms of invasiveness and metastasis of gastriccarcinoma.It is also possible that inhibition of Ets1 is apotential avenue for therapy in gastric cancer.
AIM: Ets1 proto-oncogene is a transcription factor involved in the activation of several genes of tumor invasion and metastasis. We aimed to determine the relationship betweenthe extent and intensity of Ets1 expression and patients’clinicopathological factors in gastric carcinoma. METHODS: Immunohistochemical analysis was performed for gastric tumor paraffin-embedded sections, followed by image analysis. RESULTS: Ets1 was not expressed in the normal gastricepithelium and its surrounding cells. The percentage of Ets1 expressing cells detected increased significantly in bothepitial tumor and stromal cells from high T classification, lymph node metastasis positive, clinical The level of Ets1 staining in epithelial tumor cells also reflected the degree of cell differentiation. The percentage of epithelial and stromal cells expressing Ets1was significantly correlated with the presence of lymph node metastasis (P = 0.014 and P = 0.001 respectively ) Ets1expression was not observed in tissue samples from patients with benign gastric ulcers. CONCLUSION: Ets1 protein expression in epithelial tumor cells ranked the degree of differentiation, and the percentage of Ets1 positive tumor and stromal cells correlates with lymphnode metastasis. Thus Ets1 is a valuable marker of malignant potential in terms of invasiveness and metastasis of gastriccarcinoma. It is also possible that inhibition of Ets1 is apotential avenue for therapy in gastric cancer.