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目的:运用体外培养神经元与胶质细胞相互作用的形态学指标为神经损伤性疾病的研究提供理论基础及相关的神经移植方法。方法:采用原代分离培养的方法,首先将胚胎小鼠的脊髓胶质细胞进行传代培养;然后,小鼠的脊髓神经细胞接种在胶质细胞表面进行培养,最后进行形态学观察不同时间神经元的生长状况。结果:神经元在胶质细胞表面生长活跃,突起仅局限于胶质细胞生长,并且神经突起沿着胶质细胞生长旺盛的部位生长,相反,裸区儿乎没有神经元的贴壁生长和突起的延伸,培养10天后,胶质细胞表面的神经元大部分退化死亡。结论:胚胎神经元早期可能刺激胶质细胞产生生物活性物质,这些活性物质能够促进神经元的贴壁和突起的生长,当神经元分化成熟时,这种功能丧失。
OBJECTIVE: To provide a theoretical basis and a related neural transplantation method for the study of neuronal injury by using the morphological indexes of neuronal and glial cell interactions in vitro. Methods: Primary cultured rat spinal cord glia were subcultured by primary isolation and culture. Then, the spinal cord nerve cells of mice were inoculated on the surface of glial cells for culturing. Finally, the morphological changes of neurons The growth status. RESULTS: Neurons grew actively on the surface of glial cells. Protrusion was confined to glial growth. Neurites grew along the glial growth site. In contrast, there was almost no neuronal growth and protrusion After culturing for 10 days, most neurons on glial cell surface degenerated and died. CONCLUSIONS: Early embryonic neurons may stimulate glial cells to produce biologically active substances that promote adherent and protuberant neuronal growth. This loss of function occurs when neurons differentiate and mature.