本文报告了用乳过氧化物酶法作人催乳素碘标记的体会。标记率与氯胺T法相似,但免疫活性保存良好,与抗体的结合率高,保存时间较长。当经过较长时间结合率下降后仍可再纯制,纯制后的标记激素仍有较好的免疫活性。本法标记的人催乳素作放射免疫测定,各项指标均符合要求。并对方法学和注意事项进行了讨论。 This article reports the use of lactoperoxidase for human prolactin iodine labeling experience. The labeling rate is similar to that of chloramine T method, but the immune activity is well preserved, the binding rate with the antibody is high, and the storage time is longer. After a long period of time, the binding rate still can be re-purified, and the purified labeled hormone still has better immunocompetence. Human prolactin labeled radioimmunoassay, all the indicators are in line with the requirements. The methodology and precautions were discussed.